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Invitrogen
PSPN Polyclonal Antibody, Biotin, PeproTech®
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PSPN Antibody (500-P138BT-25UG) in WB
Western Blot: To detect hPersephin by Western Blot analysis PSPN Polyclonal Antibody, Biotin (Product # 500-P138BT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hPersephin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P138BT-25UG
产品规格
种属反应
Human
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived, 20.8 kDa Recombinant Human Persephin
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
产品详细信息
AA Sequence of recombinant protein: RALSGPCQLW SLTLSVAELG LGYASEEKVI FRYCAGSCPR GARTQHGLAL ARLQGQGRAH GGPCCRPTRY TDVAFLDDRH RWQRLPQLSA AACGCGG.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Persephin. Anti-Human Persephin-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human Persephin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Persephin (500-P138) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Persephin.
Western Blot: To detect hPersephin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hPersephin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P138BT-1MG will be provided as 2 x 500 µg
靶标信息
Persephin is a disulfide-linked, homodimeric, neurotrophic factor structurally related to GDNF, artemin, and neurturin. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Persephin signals through a multicomponent receptor system, composed of RET and one of four GFR alpha (alpha1-alpha4) receptors. The GFRalpha4 was first identified in chicken, and was later shown to be the preferential binding subunit for persephin. Persephin promotes the survival of ventral midbrain dopaminergic neurons and motor neurons after sciatic nerve oxotomy, and, like GDNF, promotes ureteric bud branching. However, in contrast to GDNF and neurturin, persephin does not support the survival of peripheral neurons.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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