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Invitrogen
PTPRN Polyclonal Antibody
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PTPRN Antibody (PA5-92949) in ICC/IF
Immunofluorescence analysis of PTPRN was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PTPRN Polyclonal Antibody (Product # PA5-92949) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (... View More
产品信息
PA5-92949
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein (or fragment)
偶联物
形式
Liquid
浓度
0.77 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: STGHMILAYM EDHLRNRDRL AKEWQALCAY QAEPNTCATA QGEGNIKKNR HPDFLPYDHA RIKLKVESSP SRSDYINASP IIEHDPRMPA YIATQGPLSH TIADFWQMVW ESGCTVIVML TPLVEDGVKQ CDRYWPDEGA SLYHVYEVNL VSEHIWCEDF LVRSFYLKNV QTQETRTLTQ FHFLSWPAEG TPASTRPLLD FRRKVNKCYR GRSCPIIVHC SDGAGRTGTY ILIDMVLNRM AKGVKEIDIA ATLEHVRDQR PGLVRSKDQF EFALTAVAEE VNAILKALPQ; Positive Samples: 293T, NIH/3T3, Raji, Mouse brain, Rat brian; Cellular Location: Cell junction, Cell membrane, Cell projection, Cytoplasmic vesicle, Endosome, Membrane, Perikaryon, Single-pass type I membrane protein, axon, secretory vesicle membrane, synapse
靶标信息
IA-2 (insulinoma-associated protein 2, ICA512, PTPRN) is a member of the transmembrane protein tyrosine phosphatase (PTP) family located in secretory granules of neuroendocrine cells. The IA-2 protein is 979 amino acids in length and consists of a luminal domain, transmembrane domain, and cytoplasmic domain. Although a member of the PTP family, IA-2 lacks phosphatase activity with known substrates due to amino acid substitutions at critical sites in its PTP domain. Two paralog RPTPs, IA-2 and IA-2beta were identified as major autoantigens in type-1 diabetes mellitus. On granule exocytosis, the IA-2 cytoplasmic domain is cleaved and the resulting cytosolic fragment moves into the nucleus where it enhances the levels of phosphorylated STAT5 and STAT3, thereby inducing insulin gene transcription and granule biogenesis. IA-2 signaling enhances pancreatic beta-cell proliferation by regulating cyclins D through STATs.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 105 kDa islet cell antigen; BEM-3; Brain-enriched membrane-associated protein tyrosine phosphatase; ICA 512; ICA105; ICA512; islet cell antigen 2; Islet cell antigen 512; Islet cell autoantigen 3; protein tyrosine phosphatase, receptor type, N; protein tyrosine phosphatase, receptor-type, N; protein tyrosine phosphatase-like N; PTP IA-2; PTPLP; R-PTP-N; Receptor-type tyrosine-protein phosphatase-like N
基因别名: IA-2; IA-2/PTP; IA2; ICA3; ICA512; mIA-A; mKIAA4064; PTPRN; R-PTP-N
UniProt ID: (Human) Q16849, (Rat) Q63259
Entrez Gene ID: (Human) 5798, (Rat) 116660, (Mouse) 19275
response to reactive oxygen species
luteinization
cytokine-mediated signaling pathway
insulin secretion
response to insulin
insulin secretion involved in cellular response to glucose stimulus
response to estrogen
response to cAMP
dense core granule maturation
protein dephosphorylation
response to glucose
second-messenger-mediated signaling
dephosphorylation
