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Invitrogen
PUS10 Polyclonal Antibody
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PUS10 Antibody (PA5-90861) in WB
Western blot analysis of PUS10 in extracts of various cell lines. Samples were incubated with PUS10 Polyclonal antibody (Product # PA5-90861) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
产品信息
PA5-90861
产品规格
种属反应
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 290-529 of human PUS10 (NP_6533102)
偶联物
形式
Liquid
浓度
0.9 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: VAGRYNKYSR NLPQTPWIID GERKLESSVE ELISDHLLAV FKAESFNFSS SGREDVDVRT LGNGRPFAIE LVNPHRVHFT SQEIKELQQK INNSSNKIQV RDLQLVTREA IGHMKEGEEE KTKTYSALIW TNKAIQKKDI EFLNDIKDLK IDQKTPLRVL HRRPLAVRAR VIHFMETQYV DEHHFRLHLK TQAGTYIKEF VHGDFGRTKP NIGSLMNVTA DILELDVESV DVDWPPALDD; Positive Samples: Mouse thymus, Mouse spleen, Mouse testis, Rat thymus
靶标信息
Pseudouridylate synthases catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. PUS10 catalyzes the formation of the universal psi55 in the GC loop of transfer RNAs (Probable). Modulator of TRAIL-induced cell death via activation of procaspase 8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: coiled-coil domain containing 139; Coiled-coil domain-containing protein 139; Psi55 synthase; putative tRNA pseudouridine synthase Pus10; tRNA pseudouridine 55 synthase; tRNA pseudouridine synthase Pus10; tRNA pseudouridylate synthase; tRNA-uridine isomerase
基因别名: 2810013G11Rik; 4933435A13Rik; AU014648; C77560; Ccdc139; Pus10; RGD1306402
UniProt ID: (Mouse) Q9D3U0
Entrez Gene ID: (Rat) 305583, (Mouse) 74467
