Invitrogen

PYGL Polyclonal Antibody

Name: PYGL Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-51492

高级验证

6个已发表图片

1 篇参考文献

查看其他14个PYGL抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

PYGL Antibody in Immunocytochemistry (ICC/IF)

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PYGL Antibody (PA5-51492) in ICC/IF

Immunofluorescent staining of PYGL in human cell line U-251 MG using a PYGL Polyclonal Antibody (Product # PA5-51492) shows localization to nucleoplasm, plasma membrane and cytosol.

PYGL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PYGL Antibody in Immunohistochemistry (IHC)

Product Image Gallery

PYGL Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Relative expression of PYGL was observed in PC-3 and SK-MEL-30 in Western Blot and RNA-Seq using Anti-PYGL Rabbit Polyclonal Antibody (Product # PA5-51492). {RE}

Immunogen image

Immunogen Image PYGL Polyclonal Antibody

产品信息

PA5-51492

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:500-1:1,000

免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human PYGL. Recombinant protein control fragment (Product #RP-88883).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.10 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2646167

产品详细信息

Immunogen sequence: MRIDDVAALD KKGYEAKEYY EALPELKLVI DQIDNGFFSP KQPDLFKDII NMLFYHDRFK VFADYEAYVK CQDKVSQLYM NPKAWNTMVL KNIAASGKFS SDRTIKEYAQ NIWNVEPSD

Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 92%.

靶标信息

This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。