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PYR1/Abscisic acid receptor RCAR11 Polyclonal Antibody
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PYR1/Abscisic acid receptor RCAR11 Antibody (AS13-2634) in WB
150 µg of total protein from Arabidopsis thaliana (col-0) 2 weeks old seedlings, extracted with 50 m M Tris, 150 m M Na Cl, 05%Triton X-100,2 m M DTT, 1 m M PMSF, protease inhibitor were separated on 10% SDS-PAGE and blotted 2 h to PVDF using semi-dry. Blots were blocked with 1 x TBST with nonfat milk 5% for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilutio... View More
产品信息
AS13-2634
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PYR1 sequence.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
PYR1 encodes an intracellular abscisic acid (ABA) receptor of the PYR/PYL/RCAR family in Arabidopsis thaliana that functions in both the cytoplasm and nucleus as a key upstream sensor in ABA signaling. It is an intronless, protein-coding locus that produces a small soluble protein adopting a START/Bet v 1-like helix-grip fold with a ligand-binding cavity; PYR1 forms homodimers, and ABA binding induces conformational changes in gate/latch loops that close over the hormone and create a high-affinity interaction surface for clade A protein phosphatase 2C (PP2C) coreceptors. Functionally, ABA-bound PYR1 inhibits PP2Cs such as ABI1 and ABI2, relieving repression of downstream kinases and transcriptional programs that drive canonical ABA responses including regulation of stomatal behavior, growth modulation, and adaptive responses to water deficit and other stresses; thus, PYR1 acts as a hormone-dependent negative regulator of PP2C activity to promote ABA-responsive signaling outputs.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: PYRABACTIN RESISTANCE 1; RCAR11; regulatory component of ABA receptor 11; T6K21.50; T6K21_50
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