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Invitrogen
Paxillin Polyclonal Antibody
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Paxillin Antibody (PA5-79899) in ICC/IF
Immunocytochemistry analysis of PXN using anti-PXN antibody (Product # PA5-79899) . PXN was detected in a section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5μg/mL rabbit anti-PXN antibody (Product # PA5-79899) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize... View More
产品信息
PA5-79899
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the C-terminal of human Paxillin (456-472aa HEKDGKAYCRKDYFDMF).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide, 0.05mg thimerosal
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human 293T whole cell, human HepG2 whole cell, human HEL whole cell, rat brain tissue, mouse brain tissue. IHC: Human Lung Cancer tissue IHC-F: Rat Kidney tissue. ICC/IF: PC-3 cell, Hela cell. Flow: A549 cell.
靶标信息
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: FLJ16691; hCG_1778014; myocardial ischemic preconditioning associated protein 7; PAX-1; PAXI; Paxillin; paxillin alpha; testicular tissue protein Li 134
基因别名: AW108311; AW123232; Pax; PXN
UniProt ID: (Human) P49023, (Mouse) Q8VI36
Entrez Gene ID: (Human) 5829, (Mouse) 19303, (Rat) 360820
muscle contraction
cell adhesion
cell-matrix adhesion
signal transduction
signal complex assembly
epidermal growth factor receptor signaling pathway
transforming growth factor beta receptor signaling pathway
cellular response to reactive oxygen species
vascular endothelial growth factor receptor signaling pathway
growth hormone receptor signaling pathway
activation of MAPK activity
movement of cell or subcellular component
cytoskeleton organization
integrin-mediated signaling pathway
regulation of cell shape
peptidyl-tyrosine phosphorylation
lamellipodium assembly
substrate adhesion-dependent cell spreading
positive regulation of protein kinase activity
focal adhesion assembly
branching morphogenesis of an epithelial tube
