Invitrogen

Periplakin Polyclonal Antibody

Name: Periplakin Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-79856
Availability: InStock

查看其他8个Periplakin抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (6)

Periplakin Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

Periplakin Antibody (PA5-79856) in ICC/IF

Immunocytochemical analysis of Periplakin in A431 cells using anti-Periplakin antibody (Product # PA5-79856). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL rabbit anti-Periplakin Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1... View More

Periplakin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Periplakin Antibody in Western Blot (WB)

Periplakin Antibody in Flow Cytometry (Flow)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

Immunogen image

Immunogen Image Periplakin Polyclonal Antibody

产品信息

PA5-79856

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.25 µg/mL

免疫组化（石蜡） (IHC (P))

2-5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence at the C-terminus of human Periplakin (1664-1701aa DTGRELSPEEAHRAGLIDWNMFVKLRSQECDWEEISVK).

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2746971

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human Hela whole cell, human A549 whole cell, human SW620 whole cell, human HepG2 whole cell, human SK-OV-3 whole cell, human PANC-1 whole cell, human SGC-7801 whole cell, rat lung tissue, mouse lung tissue. IHC: human lung cancer tissue, human oesophagus squama cancer tissue, human tonsil tissue. ICC/IF: A431 cell. Flow: U20S cell.

靶标信息

The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling.

仅用于科研。不用于诊断过程。未经明确授权不得转售。