Invitrogen

Phospho-AHNAK (Ser5784) Polyclonal Antibody

Name: Phospho-AHNAK (Ser5784) Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-105822
Availability: InStock

查看其他9个AHNAK抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (4)

Phospho-AHNAK (Ser5784) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 4

Phospho-AHNAK (Ser5784) Antibody (PA5-105822) in IHC (P)

Immunohistochemistry analysis of paraffin-embedded Phospho-AHNAK (Ser5782) in mouse kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-AHNAK (Ser5782) polyclonal antibody (Product # PA5-105822) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Phospho-AHNAK (Ser5784) Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

Immunogen image

Immunogen Image Phospho-AHNAK (Ser5784) Polyclonal Antibody

产品信息

PA5-105822

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human AHNAK(Accession Q09666), corresponding to amino acid residues around phosphorylated Ser5784.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

sequential chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2817221

产品详细信息

Antibody detects endogenous levels of Desmoyokin only when phosphorylated at Ser5784.

靶标信息

AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear; it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.

仅用于科研。不用于诊断过程。未经明确授权不得转售。