Invitrogen

Phospho-BCLAF1 (Ser531) Polyclonal Antibody

Phospho-BCLAF1 (Ser531) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-BCLAF1 (Ser531) Antibody (PA5-118678) in ICC/IF

Immunocytochemistry analysis of Phospho-BCLAF1 (Ser531) in Hela cells (Forskolin 500 μm for 30 min). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-BCLAF1 (Ser531) polyclonal antibody (Product # PA5-118678) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat an... View More

Phospho-BCLAF1 (Ser531) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-BCLAF1 (Ser531) Antibody in Western Blot (WB)

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产品信息

PA5-118678

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human BCLAF1(Accession Q9NYF8), corresponding to amino acid residues around phosphorylated Ser531.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

sequential chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2903178

产品详细信息

Antibody detects endogenous levels of BCLAF1 only when phosphorylated at Ser531.

靶标信息

BCLAF1 is an intracellular, death promoting transcriptional repressor of survival genes that interacts with several members of the BCL2 family of proteins. It shows homology to the basic leucine zipper (bzip) and MYB DNA binding domains.From recent research it can be concluded that in case of apoptotic cells this protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope. When over expressed this protein induces apoptosis and can be suppressed by co-expression of BCL2 proteins. Also known as BTF, it triggers a proapoptotic signal, potentially helpful to overcome the resistance to STI571.BCLAF1 is found to express mostly in skeletal muscles.

仅用于科研。不用于诊断过程。未经明确授权不得转售。