Invitrogen

Phospho-BTK/ITK (Tyr551, Tyr511) Monoclonal Antibody (M4G3LN), eFluor™ 660, eBioscience™

Name: Phospho-BTK\/ITK (Tyr551, Tyr511) Monoclonal Antibody (M4G3LN), eFluor\u2122 660, eBioscience\u2122
Brand: Invitrogen
SKU: 50-9015-82

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说明书

实验方案

问题与答案

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
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Phospho-BTK/ITK (Tyr551, Tyr511) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Phospho-BTK/ITK (Tyr551, Tyr511) Antibody (50-9015-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue using 10 µg/mL Mouse IgG2b K Isotype Control eFluor® 660 (left) or 10 µg/mL Anti-Human/Mouse phospho-BTK/ITK (Y551/Y511) eFluor® 660 (right). Nuclei are stained with DAPI.

Phospho-BTK/ITK (Tyr551, Tyr511) Antibody in Immunohistochemistry (IHC)

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产品信息

50-9015-82

应用

建议稀释比

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

10 µg/mL

免疫细胞化学 (ICC/IF)

10 µg/mL

产品规格

种属反应

Human, Mouse

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

M4G3LN

偶联物

eFluor™ 660 eFluor™ 660 eFluor™ 660

激发/发射光谱

651/668 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2574307

产品详细信息

Description: This M4G3LN monoclonal antibody recognizes human and mouse Bruton's tyrosine kinase (also known as BTK, agammaglobulinaemia tyrosine kinase, B-cell progenitor kinase)/interleukin-2-inducible tyrosine kinase (also known as ITK, T-cell-specific kinase, Tyrosine-protein kinase Lyk) when phosphorylated on Y551 and Y511, respectively.

In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency.

Specificity of this M4G3LN clone was determined by ELISA, flow cytometry, and western blotting.

Applications Reported: This M4G3LN antibody has been reported for use in immunohistochemical staining, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This M4G3LN antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: agammaglobulinaemia tyrosine kinase; Agammaglobulinemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton agammaglobulinemia tyrosine kinase; Bruton tyrosine kinase; Bruton's tyrosine kinase; dominant-negative kinase-deficient Brutons tyrosine kinase; homolog of mouse T-cell itk/tsk; IL-2-inducible T cell kinase; IL-2-inducible T-cell kinase; IL2-inducible T-cell kinase; interleukin-2-inducible T cell kinase; Interleukin-2-inducible T-cell kinase; Kinase EMB; Kinase EMT; Kinase TLK; T-cell-specific kinase; truncated Bruton agammaglobulinemia tyrosine kinase; Tyrosine-protein kinase BTK; Tyrosine-protein kinase BTK (Brutons tyrosine kinase) (Agammaglobulinaemia tyrosine kinase) (ATK) (B cell progenitor kinase) (BPK) (Kinase EMB); tyrosine-protein kinase BTK isoform (lacking exon 13 to 17); tyrosine-protein kinase BTK isoform (lacking exon 14); Tyrosine-protein kinase ITK/TSK; Tyrosine-protein kinase Lyk; X-linked immune deficiency

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基因别名: AGMX1; AI528679; AT; ATK; BPK; BTK; EMT; IMD1; ITK; LPFS1; LYK; PSCTK1; PSCTK2; Tcsk; Tlk; Tsk; xid; XLA