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Phospho-BTK/ITK (Tyr551, Tyr511) Antibody (740047M) in WB
Western blot was performed using Phospho-ITK/BTK (Tyr551, Tyr511) Recombinant Mouse Monoclonal Antibody (M4G3LN) (Product # 740047M, 1:200) by loading whole cell extracts (60 µg lysate) of Jurkat (Lane 1) and Jurkat treated with hydrogen peroxide (1.5mM for 5 minutes) and sodium orthovanadate (0.5 mM for 5 minutes) (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel, (Product # NP0321BOX)... View More
产品信息
740047M
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG2b, kappa
Expression System
Expi293
分类
Monoclonal
类型
Antibody
克隆号
M4G3LN
偶联物
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Protein A/G
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
Recombinant mouse monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, lot-to-lot consistency, and broader immunoreactivity to diverse targets due to a larger rabbit immune repertoire.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: agammaglobulinaemia tyrosine kinase; Agammaglobulinemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton agammaglobulinemia tyrosine kinase; Bruton tyrosine kinase; Bruton's tyrosine kinase; dominant-negative kinase-deficient Brutons tyrosine kinase; homolog of mouse T-cell itk/tsk; IL-2-inducible T cell kinase; IL-2-inducible T-cell kinase; IL2-inducible T-cell kinase; interleukin-2-inducible T cell kinase; Interleukin-2-inducible T-cell kinase; Kinase EMT; T-cell-specific kinase; truncated Bruton agammaglobulinemia tyrosine kinase; Tyrosine-protein kinase BTK; tyrosine-protein kinase BTK isoform (lacking exon 13 to 17); tyrosine-protein kinase BTK isoform (lacking exon 14); Tyrosine-protein kinase ITK/TSK; Tyrosine-protein kinase Lyk
基因别名: AGMX1; AT; ATK; BPK; BTK; EMT; IMD1; ITK; LPFS1; LYK; PSCTK1; PSCTK2; XLA
UniProt ID: (Human) Q06187, (Human) Q08881
Entrez Gene ID: (Human) 695, (Human) 3702
negative regulation of cytokine production
adaptive immune response
regulation of B cell cytokine production
MyD88-dependent toll-like receptor signaling pathway
regulation of B cell apoptotic process
transcription, DNA-templated
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
I-kappaB kinase/NF-kappaB signaling
mesoderm development
peptidyl-tyrosine phosphorylation
calcium-mediated signaling
intracellular signal transduction
peptidyl-tyrosine autophosphorylation
Fc-epsilon receptor signaling pathway
B cell activation
regulation of cell proliferation
innate immune response
positive regulation of B cell differentiation
cell maturation
B cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
apoptotic signaling pathway
cytokine production
NK T cell differentiation
cellular defense response
signal transduction
activation of phospholipase C activity
interferon-gamma production
interleukin-4 production
T cell activation
T cell receptor signaling pathway
