Invitrogen

Phospho-C/EBP beta (Thr235) Recombinant Rabbit Monoclonal Antibody (3T6A7)

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问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (4)

Phospho-C/EBP beta (Thr235) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-C/EBP beta (Thr235) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Phospho-C/EBP beta (Thr235) Antibody (MA569932) in IHC (P)

Immunohistochemistry analysis of Phospho-C/EBP beta (Thr235) on paraffin-embedded Human esophagus tissue. Samples were incubated with Phospho-C/EBP beta (Thr235) recombinant monoclonal antibody (Product # MA569932) using a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0. 01 M Tris-EDTA Buffer (pH 9. 0).

Phospho-C/EBP beta (Thr235) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-C/EBP beta (Thr235) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-C/EBP beta (Thr235) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-C/EBP beta (Thr235) Antibody in Western Blot (WB)

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Phospho-C/EBP beta (Thr235) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-C/EBP beta (Thr235) on paraffin-embedded Human esophagus tissue. Samples were incubated with Phospho-C/EBP beta (Thr235) recombinant monoclonal antibody (Product # MA569932) using a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0. 01 M Tris-EDTA Buffer (pH 9. 0).

Phospho-C/EBP beta (Thr235) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-C/EBP beta (Thr235) on paraffin-embedded Mouse brain tissue. Samples were incubated with Phospho-C/EBP beta (Thr235) recombinant monoclonal antibody (Product # MA569932) using a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0. 01 M Tris-EDTA Buffer (pH 9. 0).

Phospho-C/EBP beta (Thr235) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-C/EBP beta (Thr235) on paraffin-embedded Rat brain tissue. Samples were incubated with Phospho-C/EBP beta (Thr235) recombinant monoclonal antibody (Product # MA569932) using a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0. 01 M Tris-EDTA Buffer (pH 9. 0).

Phospho-C/EBP beta (Thr235) Antibody in Western Blot (WB) — Western blot analysis of Phospho-C/EBP beta (Thr235) in MCF7 cells. Samples were probed with Phospho-C/EBP beta (Thr235) recombinant monoclonal antibody (Product # MA569932) at 1:1,000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 30 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

产品信息

MA569932

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:200-1:800

-

酶联免疫吸附实验 (ELISA)

1 µg/mL

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

3T6A7

抗原

Synthetic peptide.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.07 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3., with 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: AGP/EBP; C EBP; C/EBP BETA; C/EBP-related protein 2; CCAAT/enhancer binding protein (C/EBP), beta; CCAAT/enhancer-binding protein beta; IL-6DBP; interleukin 6-dependent DNA-binding protein; Interleukin-6-dependent-binding protein; LAP; LIP; Liver activator protein; Liver-enriched inhibitory protein; Liver-enriched transcriptional activator; Nuclear factor NF-IL6; nuclear factor of interleukin 6; nuclear factor-IL6; nuclear protein Il6; OTTMUSP00000017503; SF-B; Silencer factor B; TCF-5; Transcription factor 5; transcription factor C/EBP beta; unnamed protein product

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基因别名: C/EBP-beta; C/EBPbeta; CEBPB; CRP2; IL-6DBP; IL6DBP; LAP; LIP; NF-IL6; NF-M; Nfil6; PP9092; Sfb; TCF5

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UniProt ID: (Human) P17676, (Mouse) P28033, (Rat) P21272

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Entrez Gene ID: (Human) 1051, (Mouse) 12608, (Rat) 24253

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transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding kinase binding chromatin DNA binding histone acetyltransferase binding glucocorticoid receptor binding identical protein binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding ubiquitin-like protein ligase binding protein heterodimerization activity RNA polymerase II sequence-specific DNA binding transcription factor binding DNA-binding transcription factor binding sequence-specific double-stranded DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription factor binding basic leucine zipper transcription factor

negative regulation of transcription from RNA polymerase II promoter ovarian follicle development liver development embryonic placenta development granuloma formation transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter acute-phase response inflammatory response immune response memory positive regulation of gene expression cell differentiation neuron differentiation response to lipopolysaccharide regulation of interleukin-6 production positive regulation of interleukin-4 production mammary gland epithelial cell proliferation response to endoplasmic reticulum stress T-helper 1 cell activation negative regulation of T cell proliferation defense response to bacterium negative regulation of neuron apoptotic process fat cell differentiation regulation of cell differentiation positive regulation of fat cell differentiation positive regulation of osteoblast differentiation regulation of osteoclast differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of inflammatory response brown fat cell differentiation mammary gland epithelial cell differentiation myeloid cell development intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress positive regulation of biomineral tissue development cellular response to lipopolysaccharide cellular response to amino acid stimulus cellular response to interleukin-1 hepatocyte proliferation liver regeneration positive regulation of cold-induced thermogenesis integrated stress response signaling regulation of odontoblast differentiation positive regulation of sodium-dependent phosphate transport regulation of dendritic cell differentiation regulation of interleukin-6 biosynthetic process regulation of transcription involved in cell fate commitment positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress