Invitrogen

Phospho-CREB/ATF1 (Ser133, Ser63) Monoclonal Antibody (10E9)

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Phospho-CREB/ATF1 (Ser133, Ser63) Monoclonal Antibody (10E9)

产品信息

MA1-114

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:500
-

免疫组化（石蜡） (IHC (P))

1:50-1:500
-

免疫细胞化学 (ICC/IF)

1:20-1:200
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流式细胞分析 (Flow)

1 µg/test
-

酶联免疫吸附实验 (ELISA)

0.05 µg/mL
-

染色质免疫沉淀 实验 (ChIP)

5 µL/10^6 cells
-
产品规格

种属反应

Dog, Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

10E9

抗原

A synthetic phosphopeptide corresponding to amino acids 125-135 surrounding the Ser133 phosphorylation site of human CREB
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Size-exclusion chromatography

保存液

PBS, pH 7.2, with sucrose, PEG, 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2536825

产品详细信息

This antibody recognizes CREB protein phosphorylated at serine 133 and ATF1 phosphorylated at serine 63. The antibody was purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.

Reconstitute with 1 mL H2O (15 min, RT)

Aliquot and freeze in liquid nitrogen. Antibody can be stored frozen at -80°C for up to 1 year. Thaw aliquots at 37°C; thawed aliquots may be stored at 4°C for up to 3 months. Avoid repeated freeze/thaw cycles.

This antibody was originally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

靶标信息

Cyclic AMP response element binding protein (CREB) is a basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44/42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB appears to play an important role in learning and memory.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Activating transcription factor 1; active transcription factor CREB; cAMP-dependent transcription factor ATF-1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB-1; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; Cyclic AMP-dependent transcription factor ATF-1; Cyclic AMP-responsive element-binding protein 1; Protein TREB36; Ser133; TCR-ATF1; transactivator protein

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基因别名: 2310001E10Rik; 3526402H21Rik; ATF1; AV083133; CREB; CREB-1; CREB1; EWS-ATF1; FUS/ATF-1; TREB36

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UniProt ID: (Human) P18846, (Mouse) P81269, (Human) P16220, (Mouse) Q01147

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Entrez Gene ID: (Dog) 486545, (Human) 466, (Mouse) 11908, (Dog) 607922, (Human) 1385, (Mouse) 12912

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功能
RNA polymerase II regulatory region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding protein complex binding protein heterodimerization activity DNA binding sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding transcriptional activator activity, RNA polymerase II transcription factor binding transcription cofactor activity enzyme binding Hsp70 protein binding histone acetyltransferase binding cAMP response element binding identical protein binding arrestin family protein binding double-stranded DNA binding transcription factor binding transcription regulatory region DNA binding
参与通路
transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter protein phosphorylation transforming growth factor beta receptor signaling pathway axonogenesis lactation memory circadian rhythm regulation of cell size response to organic substance negative regulation of transcription by competitive promoter binding pituitary gland development cell differentiation mammary gland development positive regulation of transforming growth factor beta3 production secretory granule organization response to glucagon positive regulation of multicellular organism growth response to drug regulation of circadian rhythm regulation of apoptotic process positive regulation of fat cell differentiation positive regulation of osteoclast differentiation positive regulation of transcription, DNA-templated positive regulation of RNA polymerase II transcriptional preinitiation complex assembly positive regulation of transcription from RNA polymerase II promoter positive regulation of hormone secretion positive regulation of lipid biosynthetic process rhythmic process protein stabilization lung epithelium development lung saccule development Type I pneumocyte differentiation cellular response to zinc ion cellular response to growth factor stimulus regulation of transcription from RNA polymerase II promoter positive regulation of neuron projection development cellular protein complex assembly positive regulation of DNA replication response to organic cyclic compound response to cobalt ion response to hypoxia signal transduction axon guidance aging visual learning response to activity viral process chemotaxis to arachidonic acid response to nicotine cellular response to hepatocyte growth factor stimulus cellular response to platelet-derived growth factor stimulus response to antidepressant positive regulation of apoptotic process regulation of fibroblast proliferation regulation of glial cell proliferation cellular response to fatty acid positive regulation of long-term synaptic potentiation negative regulation of neuron death response to L-glutamate cellular response to nerve growth factor stimulus cellular response to insulin-like growth factor stimulus
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