Invitrogen

Phospho-Cardiac Troponin I (Ser23, Ser24) Polyclonal Antibody

Phospho-Cardiac Troponin I (Ser23, Ser24) Antibody in Western Blot (WB)

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Phospho-Cardiac Troponin I (Ser23, Ser24) Antibody (PA5-85825) in WB

Western blot analysis of Phospho-Cardiac Troponin I (Ser23, Ser24) in various tissue extracts using Phospho-Cardiac Troponin I (Ser23, Ser24) polyclonal antibody (Product # PA5-85825) using 50 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 12% SDS-PAGE.

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产品信息

PA5-85825

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

产品规格

种属反应

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Carrier-protein conjugated synthetic peptide corresponding to residues around mouse Cardiac Troponin I (phospho Ser23/Ser24).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2792961

产品详细信息

Keep as concentrated solution.

Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Cat (100%), Pig (100%), Bovine (100%).

Positive Control: mouse heart, mouse spleen, rat heart.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Cardiac Troponin I (Cardiac-specific troponin I, TnI, Troponin I) is an integral inhibitory protein in cardiac muscle that exists as part of a complex with troponin C (TnC) and troponin T (TnT). The TnT subunit of troponin binds to tropomyosin-t form, a troponin-tropomyosin complex, anchored in place by the binding of TnI to actin, within muscle thin filaments. Structural change resulting from the binding of calcium to specific sites on the regulatory TnC subunit, releases the inhibitory region of TnI from actin, anabling the attachment of the molecular motor protein myosin, allowing for muscle contraction and hence movement. The measurements of blood TnI and TnT levels is an important diagnostics indicator of heart muscle damage, and can be used to differentiate between angina and myocardial infarction in patients with chest pains. Cardiac Troponin I has also been shown to inhibit angiogenesis in vivo and in vitro. Mutations in the Cardiac Troponin I gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).

仅用于科研。不用于诊断过程。未经明确授权不得转售。