Invitrogen

Phospho-Cullin 4A (Ser40) Polyclonal Antibody

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问题与答案

抗体检测数据 (5)

Phospho-Cullin 4A (Ser40) Antibody in Immunocytochemistry (ICC/IF)

Phospho-Cullin 4A (Ser40) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-Cullin 4A (Ser40) Antibody (PA5-105313) in ICC/IF

Immunofluorescent analysis of Phospho-Cullin 4A (Ser40) in HeLa cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-Cullin 4A (Ser40) polyclonal antibody (Product # PA5-105313) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fl... View More

Phospho-Cullin 4A (Ser40) Antibody in Immunocytochemistry (ICC/IF)

Phospho-Cullin 4A (Ser40) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-Cullin 4A (Ser40) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-Cullin 4A (Ser40) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-Cullin 4A (Ser40) Antibody in Western Blot (WB)

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Phospho-Cullin 4A (Ser40) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Phospho-Cullin 4A (Ser40) in HeLa cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-Cullin 4A (Ser40) polyclonal antibody (Product # PA5-105313) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

Phospho-Cullin 4A (Ser40) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded Phospho-Cullin 4A (Ser40) in mouse kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-Cullin 4A (Ser40) polyclonal antibody (Product # PA5-105313) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Phospho-Cullin 4A (Ser40) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded Phospho-Cullin 4A (Ser40) in human heart tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-Cullin 4A (Ser40) polyclonal antibody (Product # PA5-105313) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Phospho-Cullin 4A (Ser40) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded Phospho-Cullin 4A (Ser40) in rat heart tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-Cullin 4A (Ser40) polyclonal antibody (Product # PA5-105313) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Phospho-Cullin 4A (Ser40) Antibody in Western Blot (WB) — Western blot analysis of Phospho-Cullin 4A (Ser40) in HeLa cell lysate (Lane 1: treated with phospho-blocking peptide; Lane 2: treated with non-phospho-blocking peptide). Samples were incubated with Phospho-Cullin 4A (Ser40) polyclonal antibody (Product # PA5-105313).

Immunogen image

Phospho-Cullin 4A (Ser40) Polyclonal Antibody

Immunogen Image Phospho-Cullin 4A (Ser40) Polyclonal Antibody

产品信息

PA5-105313

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human CUL4A(Accession Q13619), corresponding to amino acid residues around phosphorylated Ser40.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

sequential chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of CUL4A only when phosphorylated at Ser40.

靶标信息

CUL4A (Cullin 4A) is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CUL 4A; CUL-4A; Cul4a protein; Cullin-4A; MGC36573; MGC64071; OTTHUMP00000018753

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基因别名: 2810470J21Rik; AW495282; CUL4A; RGD1563853

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UniProt ID: (Human) Q13619, (Mouse) Q3TCH7

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Entrez Gene ID: (Human) 8451, (Mouse) 99375, (Rat) 361181

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protein binding ubiquitin protein ligase binding ubiquitin protein ligase activity ubiquitin-protein ligase

G1/S transition of mitotic cell cycle nucleotide-excision repair, DNA damage recognition nucleotide-excision repair, DNA duplex unwinding in utero embryonic development transcription-coupled nucleotide-excision repair nucleotide-excision repair, preincision complex stabilization nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 3'-to lesion nucleotide-excision repair, DNA incision, 5'-to lesion cellular response to DNA damage stimulus cell cycle arrest positive regulation of cell proliferation negative regulation of cell proliferation viral process hemopoiesis negative regulation of granulocyte differentiation nucleotide-excision repair, DNA incision somatic stem cell population maintenance DNA damage response, detection of DNA damage protein ubiquitination involved in ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process regulation of protein metabolic process global genome nucleotide-excision repair intrinsic apoptotic signaling pathway positive regulation of G1/S transition of mitotic cell cycle regulation of DNA damage checkpoint regulation of nucleotide-excision repair DNA repair ubiquitin-dependent protein catabolic process positive regulation of protein catabolic process