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Invitrogen
Phospho-DIAPH2 (Ser196) Polyclonal Antibody
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Phospho-DIAPH2 (Ser196) Antibody (PA5-143679) in WB
Western blot of Phospho-DIAPH2 (Ser196) in mDia3 expression in human Jurkat cells treated with Calyculin A (100 nM) (lanes 1-4). The blots were treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal anti-mDia3 (C-terminus) (lanes 1 & 2) and Phospho-DIAPH2 (Ser196) polyclonal antibody (Product # PA5-143679) (lanes 3 & 4).
产品信息
PA5-143679
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Phospho-mDia3 (Ser-196) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-196 in human mDia3 (Diap2). This peptide sequence is identical in rat and mouse mDia3, and the site is well conserved in human mDia1/Diap1 (Ser-171) and mDia2/Diap3 (Ser-202).
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA, 50% glycerol
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
The antibody detects a 130 kDa* protein corresponding to the apparent molecular mass of mDia3 on SDS-PAGE immunoblots of human Jurkat treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment. The antibody may also detect phosphorylated mDia1/Diap1 (Ser-171) and mDia2/Diap3 (Ser-202).
靶标信息
The product of this gene belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this gene have been linked to premature ovarian failure 2. Alternatively spliced transcript variants encoding different isoforms have been identified.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: diaphanous homolog 2; diaphanous-related formin 2; Diaphanous-related formin-2; diaphorase-2; DRF2; FLJ11167; mDia3; mDia3 isoform; Protein diaphanous homolog 2; RP11-212B22.1
基因别名: DIA; DIA2; Dia3; Diap2; DIAPH2; DRF2; E430022I22Rik; POF; POF2; RGD1564399
UniProt ID: (Human) O60879, (Mouse) O70566
Entrez Gene ID: (Human) 1730, (Mouse) 54004, (Rat) 317189
