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Invitrogen
Phospho-DOK1/DOK2 (Tyr362, Tyr337) Polyclonal Antibody
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Phospho-DOK1/DOK2 (Tyr362, Tyr337) Antibody (PA5-143681) in WB
Western blot of Phospho-DOK1/DOK2 (Tyr362, Tyr337) in Jurkat cells stimulated with calyculin A (100 nM, 30 min.) (lanes 1-6) followed by lambda phosphatase (lanes 2 & 6) or alkaline phosphatase (lane 4) treatment. The blots were probed with anti-Dok1 (lanes 1 & 2), Phospho-DOK1/DOK2 (Tyr362, Tyr337) polyclonal antibody (Product # PA5-143681) (lanes 3 & 4), and anti-Dok1 (Ser450) (lanes 5 & 6).
产品信息
PA5-143681
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Phospho-Dok1 (Tyr-362) synthetic peptide (coupled to KLH) corresponds to amino acids surrounding tyrosine 362 in human Dok1. This sequence is conserved in Dok1 from rat and mouse (Tyr-361), and has high homology to Dok2 (Tyr-337). The site is not conserved in other Dok family members.
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA, 50% glycerol
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody was cross-adsorbed to a phosphotyrosine peptide before affinity purification using phospho-Dok1 (Tyr-362) peptide. The purified antibody detects a band at 62 kDa* corresponding to Dok1 in western blots of human Jurkat cells, but does not detect this band after alkaline phosphatase treatment. Similar to Dok1 (Ser-450) antibody, this antibody also detects an 80 kDa band that has not been identified.
靶标信息
Doks are a family of adaptor proteins that include six Dok proteins (Dok1 to Dok6), which have an N-terminal pleckstrin homology domain, a central phosphotyrosine binding domain, and a C-terminal region containing multiple tyrosine residues. When phosphorylated, these tyrosines can serve as docking sites for SH2 domain-containing proteins. Dok1 (p62dok) has been shown to bind Ras-GAP, Nck, and Csk. Several tyrosine phosphorylation sites have been identified for Dok1. One site, Tyr-362 (Tyr-361 mouse), is phosphorylated by c-Abl, is required for Nck binding, and may be critical for filopodia formation during fibroblast spreading on fibronectin. Alternatively, Dok1 activity is also regulated by serine phosphorylation. IkappaB Kinase beta phosphorylates several serine sites including Ser-450 in vitro, and TNFalpha, IL-1, and radiation treatment lead to phosphorylation of Ser-443, Ser-446, and Ser-450 in vivo. Phosphorylation of these serine sites may be required for Dok-mediated inhibition of MAPK signaling and stimulation of cell motility.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Docking protein 1; docking protein 1, 62kDa (downstream of tyrosine kinase 1); Downstream of tyrosine kinase 1; downstream of tyrosine kinase-1; p62(dok); p62DOK; pp62
基因别名: AW557123; Dok; Dok-1; DOK1; P62DOK; pp62
UniProt ID: (Human) Q99704, (Rat) Q4QQV2, (Mouse) P97465
Entrez Gene ID: (Human) 1796, (Rat) 312477, (Mouse) 13448
