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Invitrogen
Phospho-EEF2 (Thr56) Polyclonal Antibody
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Phospho-EEF2 (Thr56) Antibody (PA5-97417) in WB
Western blot analysis of Phospho-EEF2 (Thr56) in extracts of various cell lines. Samples were incubated with Phospho-EEF2 (Thr56) Polyclonal antibody (Product # PA5-97417) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. NIH/3T3 cells were treated by Forskolin (30 µM) for 30 minutes after serum-starvation overnight. C6 cells were treated b... View More
产品信息
PA5-97417
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic phosphorylated peptide around T56 of human Phospho-eEF2-T56 (NP_0019521)
偶联物
形式
Liquid
浓度
0.50 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: TRFTD; Positive Samples: NIH/3T3, C6; Cellular Location: Cytoplasm, Nucleus
靶标信息
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: EF-2; Elongation factor 2; elongation factor-2; polypeptidyl-tRNA translocase
基因别名: EEF-2; EEF2; EF-2; EF2; SCA26
UniProt ID: (Human) P13639, (Mouse) P58252, (Rat) P05197
Entrez Gene ID: (Human) 1938, (Mouse) 13629, (Rat) 29565
p53 binding
translation elongation factor activity
GTPase activity
protein binding
GTP binding
5S rRNA binding
protein kinase binding
ribosome binding
poly(A) RNA binding
actin filament binding
cadherin binding involved in cell-cell adhesion
nucleotide binding
translation activator activity
translation elongation factor
hematopoietic progenitor cell differentiation
response to ischemia
skeletal muscle contraction
translational elongation
aging
glial cell proliferation
response to fluoxetine
peptidyl-diphthamide biosynthetic process from peptidyl-histidine
response to estradiol
response to endoplasmic reticulum stress
skeletal muscle cell differentiation
response to hydrogen peroxide
response to ethanol
positive regulation of translation
response to folic acid
cell-cell adhesion
cellular response to brain-derived neurotrophic factor stimulus
positive regulation of cytoplasmic translation
translation
