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Invitrogen
Phospho-ERK1/ERK2 (Thr185, Tyr187) Recombinant Rabbit Monoclonal Antibody (15H10L7)
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Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody (700012) in ICC/IF
Immunofluorescence analysis of Phosph-ERK1/2 (Thr 185,Tyr187) was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ERK2 Rabbit monoclonal Antibody (Product # 700012) at 5 µg/mL in 0.1% BSA and incubated over... View More
产品信息
700012
应用
建议稀释比
已发表文章
产品规格
种属反应
Dog,
Human,
Rat
已发表种属
C. elegans,
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
15H10L7
抗原
A peptide corresponding to amino acids 198-208 and 181-191 of P27361 and P28482, respectively.
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
700012 has successfully been used in western blot to detect phospho-ERK1/2 in TPA-treated HeLa, NRK and MDCK cell lysates.
This antibody is predicted to react with bovine, chicken, chimpanzee, mouse, rat, Xenopus and zebrafish based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ERK-1; ERK-2; erk1 erk2; ERK1b; ERT1; ERT2; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; extracellular-signal-regulated kinase 2; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; Microtubule-associated protein 2 kinase; mitogen-activated 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; MNK1; p42; p42 MAP Kinase; p42-MAPK; p44-ERK1; p44-MAPK; protein tyrosine kinase ERK2
基因别名: ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; HS44KDAP; HUMKER1A; Mapk; MAPK1; MAPK2; MAPK3; MNK1; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3
UniProt ID: (Human) P28482, (Human) P27361, (Rat) P63086, (Rat) P21708
Entrez Gene ID: (Human) 5594, (Dog) 477575, (Human) 5595, (Dog) 100855877, (Rat) 116590, (Rat) 50689
phosphotyrosine binding
DNA binding
protein serine/threonine kinase activity
MAP kinase activity
protein binding
ATP binding
transcription factor binding
RNA polymerase II carboxy-terminal domain kinase activity
kinase activity
phosphatase binding
mitogen-activated protein kinase kinase kinase binding
identical protein binding
scaffold protein binding
protein kinase activity
protein kinase binding
non-receptor serine/threonine protein kinase
MAPK cascade
activation of MAPK activity
MAPK import into nucleus
transcription, DNA-templated
protein phosphorylation
apoptotic process
chemotaxis
response to stress
cellular response to DNA damage stimulus
cell cycle
signal transduction
chemical synaptic transmission
axon guidance
positive regulation of cell proliferation
fibroblast growth factor receptor signaling pathway
response to toxic substance
positive regulation of peptidyl-threonine phosphorylation
regulation of phosphatidylinositol 3-kinase signaling
viral process
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
sensory perception of pain
cytosine metabolic process
platelet activation
positive regulation of cell migration
thyroid gland development
regulation of protein stability
lipopolysaccharide-mediated signaling pathway
positive regulation of telomere maintenance via telomerase
regulation of stress-activated MAPK cascade
mammary gland epithelial cell proliferation
Fc-epsilon receptor signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
ERBB signaling pathway
outer ear morphogenesis
response to exogenous dsRNA
response to estrogen
negative regulation of cell differentiation
positive regulation of translation
positive regulation of transcription, DNA-templated
thymus development
T cell receptor signaling pathway
B cell receptor signaling pathway
regulation of sequence-specific DNA binding transcription factor activity
regulation of cytoskeleton organization
positive regulation of telomerase activity
Bergmann glial cell differentiation
long-term synaptic potentiation
face development
JAK-STAT cascade involved in growth hormone signaling pathway
lung morphogenesis
trachea formation
labyrinthine layer blood vessel development
cardiac neural crest cell development involved in heart development
ERK1 and ERK2 cascade
response to epidermal growth factor
caveolin-mediated endocytosis
regulation of Golgi inheritance
cellular response to granulocyte macrophage colony-stimulating factor stimulus
regulation of cellular response to heat
positive regulation of telomere capping
regulation of early endosome to late endosome transport
positive regulation of protein phosphorylation
transcription initiation from RNA polymerase I promoter
protein complex assembly
DNA damage induced protein phosphorylation
phosphorylation
arachidonic acid metabolic process
BMP signaling pathway
positive regulation of cyclase activity
positive regulation of histone phosphorylation
positive regulation of histone acetylation
peptidyl-tyrosine autophosphorylation
positive regulation of transcription from RNA polymerase II promoter
cartilage development
positive regulation of ERK1 and ERK2 cascade
interleukin-1-mediated signaling pathway
cellular response to mechanical stimulus
positive regulation of xenophagy
negative regulation of apolipoprotein binding
organ morphogenesis
response to lipopolysaccharide
intracellular signal transduction
cellular response to organic substance
