Invitrogen

Phospho-GSK3 alpha/beta (Ser21, Ser9) Recombinant Rabbit Monoclonal Antibody (2J1P4)

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说明书
实验方案
问题与答案
技术支持
Phospho-GSK3 alpha/beta (Ser21, Ser9) Recombinant Rabbit Monoclonal Antibody (2J1P4)

产品信息

MA5-51516

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:500-1:1,000
-

酶联免疫吸附实验 (ELISA)

1 µg/mL
-
产品规格

种属反应

Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

2J1P4

抗原

A synthetic phosphorylated peptide around S21&S9 of human GSK3 alpha/GSK3 beta (NP_063937.2/NP_002084.2).
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.83 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

内含物

0.05% ProClin 300

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_3093953

产品详细信息

Immunogen sequence: TSSFA

靶标信息

Glycogen synthase kinase-3 (GSK3) is a protein kinase that was originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3's substrate specificity is unique in that phosphorylation of the substrate only occurs if a phosphoserine or phosphotyrosine is present - four residues C-terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, GSK3 alpha and GSK3 beta, and they are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3alpha) by protein kinase B (PKB) causes its inactivation and is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor-1 (HSF-1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Glycogen synthase kinase-3 alpha; Glycogen synthase kinase-3 beta; GSK-3 alpha; GSK-3 beta; gsk-3-alpha; Serine/threonine-protein kinase GSK3A; Serine/threonine-protein kinase GSK3B

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基因别名: 2700086H06Rik; 7330414F15Rik; 8430431H08Rik; C86142; GSK-3; GSK-3beta; GSK3; Gsk3a; Gsk3b

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UniProt ID: (Mouse) Q2NL51, (Mouse) Q9WV60

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Entrez Gene ID: (Mouse) 606496, (Mouse) 56637

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功能
nucleotide binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase A catalytic subunit binding tau-protein kinase activity RNA polymerase II transcription factor binding p53 binding integrin binding beta-catenin binding transcription factor binding protein kinase binding ubiquitin protein ligase binding ionotropic glutamate receptor binding tau protein binding NF-kappaB binding non-receptor serine/threonine protein kinase
参与通路
regulation of systemic arterial blood pressure cardiac left ventricle morphogenesis carbohydrate metabolic process glycogen metabolic process regulation of gene expression by genetic imprinting protein phosphorylation nervous system development insulin receptor signaling pathway negative regulation of signal transduction positive regulation of peptidyl-threonine phosphorylation Wnt signaling pathway phosphorylation cell migration positive regulation of cAMP biosynthetic process negative regulation of TOR signaling cellular response to insulin stimulus positive regulation of peptidyl-serine phosphorylation cellular response to interleukin-3 proteasome-mediated ubiquitin-dependent protein catabolic process hypermethylation of CpG island positive regulation of heart contraction positive regulation of transcription from RNA polymerase II promoter negative regulation of glucose import negative regulation of insulin receptor signaling pathway negative regulation of transferase activity negative regulation of cell growth involved in cardiac muscle cell development cellular response to lithium ion cellular response to organic cyclic compound positive regulation of adrenergic receptor signaling pathway extrinsic apoptotic signaling pathway in absence of ligand positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway regulation of mitophagy positive regulation of protein targeting to mitochondrion positive regulation of glycogen (starch) synthase activity re-entry into mitotic cell cycle epithelial to mesenchymal transition positive regulation of cell-matrix adhesion protein export from nucleus ER overload response cytoskeleton organization establishment or maintenance of cell polarity multicellular organism development axonogenesis myoblast fusion circadian rhythm cell proliferation organ morphogenesis negative regulation of cardiac muscle hypertrophy regulation of neuron projection development negative regulation of neuron projection development negative regulation of neuron maturation myotube differentiation peptidyl-serine phosphorylation hippocampus development establishment of cell polarity cell differentiation negative regulation of protein complex assembly positive regulation of protein complex assembly negative regulation of protein binding positive regulation of protein binding positive regulation of proteasomal ubiquitin-dependent protein catabolic process regulation of microtubule-based process protein localization to microtubule intracellular signal transduction positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of MAP kinase activity positive regulation of GTPase activity canonical Wnt signaling pathway involved in positive regulation of apoptotic process fat cell differentiation positive regulation of axon extension protein autophosphorylation positive regulation of protein export from nucleus regulation of neuronal synaptic plasticity rhythmic process negative regulation of dendrite morphogenesis negative regulation of NFAT protein import into nucleus canonical Wnt signaling pathway superior temporal gyrus development cellular response to mechanical stimulus positive regulation of stem cell differentiation
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