Hamburger Menu Button
Thermo Fisher Scientific Logo
登录
没有账号?建立账号 注册
  • 产品
    • 抗体
    • 引物合成
    • GeneArt Gene Synthesis Instant Designer
    • TaqMan Assay and Array Search
    • PCR 设备和耗材
    • 色谱柱和纯化柱
    • 实验室离心机
    • 分子生物学试剂和试剂盒
    • 实验室塑料耗材及用品
    • 查看所有产品分类
  • 应用和技术
    • 色谱
    • 细胞转染和培养
    • 实时荧光定量PCR
    • 质谱
    • 实验室塑料耗材及用品
    • 电子显微镜
    • 蛋白质生物学
    • PCR
    • RNAi
    • 环境
    • 查看所有应用和技术
  • 服务
    • 定制服务
    • 企业级实验室信息化服务
    • 金融&租赁服务
    • 仪器服务
    • OEM和商业供应
    • 培训服务
    • 360° CDMO 和 CRO 服务
    • CDMO 服务
    • 临床试验 CRO 服务
    • Unity Lab Services
    • 查看所有帮助和支持
  • 帮助和支持
    • 学习中心
    • 联系我们
    • 更改国家或地区
    • 查看所有帮助和支持
  • 热门产品
    • TaqMan Real-Time PCR Assays
      TaqMan 实时荧光定量 PCR 试剂盒
    • Antibodies
      抗体
    • Oligos, Primers & Probes
      寡核苷酸、引物、探针
    • GeneArt Gene Synthesis
      GeneArt 基因合成
    • Cell Culture Plastics
      细胞培养耗材
  • Who We Serve
    • 抗体治疗解决方案
    • 细胞与基因治疗解决方案
    • 学术科研
    • 工业与应用
  • 联系我们
  • 货号直购
  • 订单查询
  • 查找文件&证书
Thermo Fisher Scientific Logo

Search

搜索全部或下列指定分类
Search button
          • 联系我们
          • 货号直购
          • 登录
            登录
            没有账号?建立账号 注册
            • 我的账号
            • 订单
            • 会员中心
            • 账单及发票查询
            • 定制产品及项目
          • Primary Antibodies ›
          • GSK3 alpha/beta Antibodies

          Invitrogen

          Phospho-GSK3 alpha/beta (Tyr216, Tyr279) Recombinant Rabbit Monoclonal Antibody (23GB4380)

          高级验证
          查看其他21个GSK3 alpha/beta抗体

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

          说明书
          Certificates
          实验方案
          问题与答案
          技术支持
          说明书
          Certificates
          实验方案
          问题与答案
          技术支持
          说明书
          Certificates
          实验方案
          问题与答案
          技术支持

          Cite Phospho-GSK3 alpha/beta (Tyr216, Tyr279) Recombinant Rabbit Monoclonal Antibody (23GB4380)

          Additional Information:
          {{banner.description}}
          {{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
          • 抗体检测数据 (3)
          • 高级验证 (1)
          Phospho-GSK3 alpha/beta (Tyr216, Tyr279) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Phospho-GSK3 alpha/beta (Tyr216, Tyr279) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 4

          {{ $ctrl.currentElement.advancedVerification.fullName }}
          {{ $ctrl.currentElement.advancedVerification.text }}

          Phospho-GSK3 alpha/beta (Tyr216, Tyr279) Antibody (MA5-53736) in ICC/IF

          Immunocytochemistry analysis of GSK3 alpha/beta in C2C12 cells. Nuclei were stained blue with DAPI; GSK3 alpha/beta recombinant monoclonal antibody (Product # MA5-53736) with a dilution of 1:1,000 was stained magenta with Alexa Fluor 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

          {{$ctrl.videoDesc}}

          {{$ctrl.videoLongDesc}}

          Phospho-GSK3 alpha/beta (Tyr216, Tyr279) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-GSK3 alpha/beta (Tyr216, Tyr279) Antibody in Western Blot (WB)
          Phospho-GSK3 alpha/beta (Tyr216, Tyr279) Antibody in Flow Cytometry (Flow)
          Phospho-GSK3 alpha/beta (Tyr216, Tyr279) Antibody

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Phospho-GSK3 alpha/beta (Tyr216, Tyr279) Recombinant Rabbit Monoclonal Antibody (23GB4380)

          产品信息

          MA5-53736

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:1,000-1:5,000
          -

          免疫细胞化学 (ICC/IF)

          1:1,000
          -

          流式细胞分析 (Flow)

          1:2,000
          -
          产品规格

          种属反应

          Human, Mouse, Rat

          宿主/亚型

          Rabbit / IgG

          Expression System

          HEK293F

          分类

          Recombinant Monoclonal

          类型

          Antibody

          克隆号

          23GB4380

          抗原

          A synthesized peptide derived from human Phospho-GSK3 (alpha+beta)(Tyr216+Tyr279) (272-286AA).
          3D表位/免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          0.6 mg/mL

          规格

          60 µg

          纯化类型

          Affinity chromatography

          保存液

          PBS, pH 7.4, with 50% glycerol

          内含物

          0.02% sodium azide

          保存条件

          -20°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_3248207

          靶标信息

          Glycogen synthase kinase-3 (GSK3) is a protein kinase that was originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3's substrate specificity is unique in that phosphorylation of the substrate only occurs if a phosphoserine or phosphotyrosine is present - four residues C-terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, GSK3 alpha and GSK3 beta, and they are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3alpha) by protein kinase B (PKB) causes its inactivation and is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor-1 (HSF-1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          篇参考文献 (0)

          您是否在文献中引用过该产品?请点击下方按钮邮件告知我们。
          引用该产品

          生物信息学

          蛋白别名: FA; factor A; GSK-3 alpha; GSK-3 beta; gsk-3-alpha; GSK3B; kinase; mGSK3beta; serine/threonine-protein kinase GSK3A; serine/threonine-protein kinase GSK3B; unnamed protein product

          查看更多 收起

          基因别名: 2700086H06Rik; 7330414F15Rik; 8430431H08Rik; GSK-3; GSK-3beta; GSK3

          查看更多 收起

          UniProt ID: (Human) P49840, (Human) P49841, (Rat) P18265, (Mouse) Q2NL51, (Mouse) Q9WV60, (Rat) P18266

          查看更多 收起

          Entrez Gene ID: (Human) 2931, (Human) 2932, (Rat) 50686, (Mouse) 606496, (Mouse) 56637, (Rat) 84027

          查看更多 收起

          功能
          protein kinase activity protein serine/threonine kinase activity receptor binding protein binding ATP binding protein kinase A catalytic subunit binding tau protein binding tau-protein kinase activity protein serine kinase activity protease binding p53 binding beta-catenin binding kinase activity protein kinase binding ubiquitin protein ligase binding dynactin binding NF-kappaB binding RNA polymerase II sequence-specific DNA binding transcription factor binding dynein complex binding scaffold protein binding protein serine/threonine kinase binding integrin binding ionotropic glutamate receptor binding
          参与通路
          regulation of systemic arterial blood pressure cardiac left ventricle morphogenesis protein phosphorylation insulin receptor signaling pathway positive regulation of autophagy positive regulation of gene expression positive regulation of peptidyl-threonine phosphorylation regulation of neuron projection development cell migration cell differentiation positive regulation of protein ubiquitination lipopolysaccharide-mediated signaling pathway negative regulation of TOR signaling positive regulation of proteasomal ubiquitin-dependent protein catabolic process cellular response to insulin stimulus positive regulation of peptidyl-serine phosphorylation cellular response to interleukin-3 proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of neuron apoptotic process positive regulation of heart contraction positive regulation of transcription from RNA polymerase II promoter negative regulation of glucose import negative regulation of insulin receptor signaling pathway negative regulation of cell growth involved in cardiac muscle cell development regulation of microtubule cytoskeleton organization cellular response to lithium ion cellular response to glucocorticoid stimulus positive regulation of adrenergic receptor signaling pathway negative regulation of canonical Wnt signaling pathway extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway autosome genomic imprinting positive regulation of substrate adhesion-dependent cell spreading positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway regulation of mitophagy positive regulation of beta-amyloid formation positive regulation of establishment of protein localization to mitochondrion negative regulation of dendrite development epithelial to mesenchymal transition positive regulation of cell-matrix adhesion heart valve development glycogen metabolic process ER overload response mitochondrion organization meiosis I circadian rhythm negative regulation of signal transduction negative regulation of gene expression negative regulation of epithelial to mesenchymal transition positive regulation of protein processing Wnt signaling pathway peptidyl-serine phosphorylation viral protein processing hippocampus development establishment of cell polarity maintenance of cell polarity negative regulation of cell migration regulation of axon extension neuron projection development negative regulation of protein complex assembly positive regulation of protein complex assembly positive regulation of protein binding positive regulation of type I interferon production regulation of protein localization regulation of microtubule-based process response to endoplasmic reticulum stress intracellular signal transduction regulation of circadian rhythm regulation of apoptotic process negative regulation of apoptotic process protein kinase B signaling positive regulation of cell differentiation negative regulation of osteoblast differentiation negative regulation of glycogen biosynthetic process positive regulation of cilium assembly positive regulation of protein catabolic process negative regulation of smoothened signaling pathway protein autophosphorylation regulation of protein export from nucleus positive regulation of protein export from nucleus regulation of dendrite morphogenesis regulation of axonogenesis canonical Wnt signaling pathway excitatory postsynaptic potential intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress negative regulation of calcineurin-NFAT signaling cascade superior temporal gyrus development cellular response to retinoic acid presynaptic modulation of chemical synaptic transmission neuron projection organization regulation of microtubule anchoring at centrosome beta-arrestin-dependent dopamine receptor signaling pathway regulation of cellular response to heat negative regulation of protein localization to nucleus regulation of long-term synaptic potentiation negative regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of protein localization to cilium negative regulation of TORC2 signaling negative regulation of dopaminergic neuron differentiation cellular response to beta-amyloid negative regulation of protein localization to centrosome positive regulation of protein localization to centrosome positive regulation of establishment of protein localization negative regulation of type B pancreatic cell development negative regulation of glycogen (starch) synthase activity positive regulation of cardiac muscle cell differentiation negative regulation of mesenchymal stem cell differentiation regulation of cell growth negative regulation of cardiac muscle hypertrophy negative regulation of developmental process regulation of striated muscle cell differentiation G1/S transition of mitotic cell cycle re-entry into mitotic cell cycle cytoplasmic translational initiation protein export from nucleus cytoskeleton organization establishment or maintenance of cell polarity regulation of mitotic cell cycle axonogenesis myoblast fusion organ morphogenesis response to zinc ion positive regulation of mitochondrial membrane potential negative regulation of neuron projection development negative regulation of neuron maturation myotube differentiation phosphorylation positive regulation of cell migration SCF-dependent proteasomal ubiquitin-dependent protein catabolic process negative regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of osteoblast proliferation negative regulation of smooth muscle cell apoptotic process protein localization to microtubule cellular response to hepatocyte growth factor stimulus hepatic stellate cell activation TORC2 signaling positive regulation of apoptotic process negative regulation of MAP kinase activity fat cell differentiation regulation of osteoblast differentiation positive regulation of osteoclast differentiation positive regulation of translation positive regulation of axon extension negative regulation of synaptic growth at neuromuscular junction positive regulation of synaptic growth at neuromuscular junction bone remodeling regulation of neuronal synaptic plasticity positive regulation of smooth muscle cell proliferation negative regulation of dendrite morphogenesis negative regulation of nitric-oxide synthase activity positive regulation of protein kinase B signaling cell growth involved in cardiac muscle cell development cellular response to mechanical stimulus positive regulation of osteoclast proliferation hepatocyte apoptotic process regulation of neurotransmitter receptor localization to postsynaptic specialization membrane regulation of modification of postsynaptic structure response to methamphetamine hydrochloride conditioned place preference negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of excitatory postsynaptic potential positive regulation of DNA biosynthetic process positive regulation of stem cell differentiation negative regulation of neuron migration response to xenobiotic stimulus response to activity response to estradiol response to insulin cellular response to erythropoietin cellular response to hydrogen peroxide cellular response to iron(II) ion response to epinephrine response to L-glutamate cellular response to dopamine response to insulin-like growth factor stimulus response to ultrasound response to angiotensin
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          guarantee_icon

          性能保障

          只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符,您即可享受免费退换货服务。*

          了解更多
          help_icon

          随时为您服务

          获取常见问题的专家解答,或者直接联系我们的技术专家,咨询应用、设备、常规产品使用问题。

          联系技术支持
          订购 Plus Icon Minus Icon
          • 订单状态查询
          • 订单支持
          • 货号直购
          • 现货供应中心
          • 电子采购
          支持 Plus Icon Minus Icon
          • 帮助&支持
          • 联系我们 - 400 820 8982
          • 技术支持中心
          • 查找文件&证书
          • 报告网站问题
          资源 Plus Icon Minus Icon
          • 学习中心
          • 促销
          • 活动&研讨会
          • 社交媒体
          关于赛默飞 Plus Icon Minus Icon
          • 关于我们
          • 招聘 招聘
          • 投资者关系 投资者关系
          • 新闻 新闻
          • 社会责任 社会责任
          • 商标
          • 政策和通知
          品牌 Plus Icon Minus Icon
          • Thermo Scientific
          • Applied Biosystems
          • Invitrogen
          • Gibco
          • Ion Torrent
          • Unity Lab Services
          • Patheon
          • PPD
          • 服务条款
          • 隐私政策
          • 定价和运输保险政策
          © 2006-2026 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
          China flag icon
          China
          本网站销售的所有产品均不得用于人类或动物之临床诊断或治疗,仅可用于工业或者科研等非医疗目的。
             电子营业执照 | 经营证照公示 | ICP备案号:沪ICP备11003924号 | 沪公网安备 31011502006935号

          Your items have has been added!


          Host server : magellan-search-green-dd86746ff-nmlcr:80/100.66.75.251:80.
          git-commit: 04e44c58f20fafe66519586ffe6db5d8a90e52ce
          git-url: https://github.com/thermofisher/magellan-search
          git-branch: release/2.53.1-Offline