Invitrogen

Phospho-Histone H2A.X (Ser139) Monoclonal Antibody (3F2)

Name: Phospho-Histone H2A.X (Ser139) Monoclonal Antibody (3F2)
Brand: Invitrogen
SKU: MA1-2022

高级验证

13个已发表图片

44 篇参考文献

查看其他76个Histone H2A.X抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Phospho-Histone H2A.X (Ser139) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-Histone H2A.X (Ser139) Antibody (MA1-2022) in ICC/IF

Immunofluorescence staining using Product # MA1-2022, treatment with paraquat and iron induces MnSOD and Phosphorylation of H2AX in RAW 264.7 macrophages. Cells were treated for 20 hours with paraquat (500 µM) and iron (200 µg/mL) and stained with anti-Phospho-H2AX antibody.

Phospho-Histone H2A.X (Ser139) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-Histone H2A.X (Ser139) Antibody in Western Blot (WB)

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Immunogen image

Immunogen Image Phospho-Histone H2A.X (Ser139) Monoclonal Antibody (3F2)

产品信息

MA1-2022

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

查看11篇已发表文章 11篇已发表文章

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:400

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

Assay-dependent

查看22篇已发表文章 22篇已发表文章

流式细胞分析 (Flow)

1 µg / 10^6 cells

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酶联免疫吸附实验 (ELISA)

Assay-dependent

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其他PubMed (Misc)

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产品规格

种属反应

Human, Mouse

已发表种属

Bovine, C. elegans, Human, Mouse, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

3F2

抗原

Synthetic peptide sequence surrounding phosphorylated Ser140

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_559491

产品详细信息

MA1-2022 was used in IHC to successfully detect H2A.X pSer140 in postnatal mouse lung section.

MA1-2022 detects human and mouse phosphorylated H2AX.

MA1-2022 has been used successfully in Western blot, immunofluorescence, and ELISA procedures. By Western blot this antibody detects ~17 kDa protein representing phosphorylated H2AX in gamma irradiated HeLa cell lysate. In immunofluorescence procedures, MA1-2022 recognizes phosphorylated H2AX in gamma irradiated HeLa cells. ELISA of phosphorylated H2AX can also be performed using MA1-2022. MA1-2022 was used in IHC to successfully detect H2A.X pSer140 in postnatal mouse lung section.

The MA1-2022 immunogen is a synthetic peptide sequence surrounding phosphorylated Ser140. Some references call Ser140, by the name Ser139.

This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

靶标信息

Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner. Structurally, H2A.x contains 143 amino acid residues. Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA. The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA. Histone H2A.X maps to the 11q23.2-q23.3 region of the human chromosome. Histone H2A.x contributes to histone-formation and therefore the structure of DNA. Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.

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