Invitrogen

Phospho-Histone H3.3 (Ser31) Recombinant Rabbit Monoclonal Antibody (25GB2465)

Name: Phospho-Histone H3.3 (Ser31) Recombinant Rabbit Monoclonal Antibody (25GB2465)
Brand: Invitrogen
SKU: MA570173

查看其他18个Histone H3.3抗体

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

抗体检测数据 (5)

Phospho-Histone H3.3 (Ser31) Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 5

Phospho-Histone H3.3 (Ser31) Antibody (MA570173) in ICC/IF

Immunocytochemistry analysis of Phospho-Histone H3.3 (Ser31) in HepG2 cells. Sample was incubated with Phospho-Histone H3.3 (Ser31) monoclonal antibody (Product # MA570173) at a dilution of 1:1,000. Nuclei were stained blue with DAPI and Alexa Fluor 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Phospho-Histone H3.3 (Ser31) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-Histone H3.3 (Ser31) Antibody in Western Blot (WB)

Phospho-Histone H3.3 (Ser31) Antibody in Flow Cytometry (Flow)

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Immunogen image

Immunogen Image Phospho-Histone H3.3 (Ser31) Recombinant Rabbit Monoclonal Antibody (25GB2465)

产品信息

MA570173

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000

免疫组化（石蜡） (IHC (P))

1:200

免疫细胞化学 (ICC/IF)

1:1,000

流式细胞分析 (Flow)

1:2,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293F

分类

Recombinant Monoclonal

类型

Antibody

克隆号

25GB2465

抗原

A synthesized peptide derived from human (24-38) Phospho-Histone H3.3 (S31).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Dry ice (international)

靶标信息

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

仅用于科研。不用于诊断过程。未经明确授权不得转售。