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Invitrogen
Phospho-IRF3 (Ser396) Monoclonal Antibody (E.875.8)
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Phospho-IRF3 (Ser396) Antibody (MA5-14947) in WB
Western blot was performed using Phospho-IRF3 (Ser396) Monoclonal Antibody (E.875.8) (Product # MA5-14947) and a 47 kDa band corresponding to Phospho-IRF3 was observed. Whole cell extracts (30 µg) of RAW 264.7 untreated cells (Lane 1), RAW 264.7 cells treated with 100 ng/mL LPS for 1 hour (Lane 2) and RAW 264.7 cells pre-treated with 1 µM Bx795 for 30 minutes followed by 100 ng/mL LPS for 1 hour (Lane 3) we... View More
产品信息
MA5-14947
产品规格
种属反应
Human,
Mouse
已发表种属
C. elegans,
Human,
Pig
宿主/亚型
Rabbit
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
E.875.8
抗原
Synthetic phosphopeptide corresponding to residues surrounding pSer396 of human IRF-3
偶联物
形式
Liquid
浓度
46 µg/mL
纯化类型
Affinity chromatography
保存液
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
内含物
<0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
It is not recommended to aliquot this antibody.
靶标信息
IFR3 (interferon regulatory factor 3) is a key transcriptional regulator of type I interferon (IFN)-dependent immune responses. It plays a critical role in the innate immune response against DNA and RNA viruses. IFR3 regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. It acts as a more potent activator of the IFN-beta gene than the IFN-alpha gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. IFR3 is found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA, or toll-like receptor signaling. It is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Mutations in the gene can result in encephalopathy, acute, infection-induced, herpes-specific 7.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Interferon regulatory factor 3; IRF-3; IRF3 (phospho S386); IRF3 (pS386); IRF3 (pSer386); IRF3 phospho; IRF3 phospho S386; IRF3 phospho Ser386; phospho IRF3
基因别名: C920001K05Rik; IIAE7; IRF-3; IRF3
UniProt ID: (Human) Q14653, (Mouse) P70671
Entrez Gene ID: (Human) 3661, (Mouse) 54131
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription cofactor activity
protein binding
identical protein binding
protein homodimerization activity
regulatory region DNA binding
transcription factor activity, sequence-specific DNA binding
transcription regulatory region DNA binding
winged helix/forkhead transcription factor
negative regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
apoptotic process
cellular response to DNA damage stimulus
lipopolysaccharide-mediated signaling pathway
negative regulation of type I interferon production
positive regulation of type I interferon production
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
TRIF-dependent toll-like receptor signaling pathway
MDA-5 signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
response to exogenous dsRNA
type I interferon biosynthetic process
positive regulation of cytokine secretion
defense response to virus
interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
positive regulation of type I interferon-mediated signaling pathway
cellular response to dsRNA
macrophage apoptotic process
programmed necrotic cell death
immune system process
transcription, DNA-templated
regulation of transcription, DNA-templated
response to bacterium
response to lipopolysaccharide
innate immune response
negative regulation of interferon-beta biosynthetic process
negative regulation of defense response to virus by host
cellular response to lipopolysaccharide
