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Phospho-IRS1 (Ser312) Antibody (710778) in ICC/IF
Immunofluorescence was performed on MCF7 cells treated with IGF (100 ng/mL/20 min). These cells were permeabilized and fixed for detection of IRS (pS312) using Anti-IRS (pS312) Recombinant Rabbit Superclonal™ Antibody (Product # 710778, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 0.4 µg/mL, 1:2500). Panel a) shows... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
710778
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
18HCLC
抗原
Phosphopeptide corresponding to Human IRS1 Ser312 (aa 309-318)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
PBS, pH 7.2-7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
This antibody is predicted to react with Monkey, Bovine, Horse and Cat.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
靶标信息
IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.
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生物信息学
蛋白别名: Insulin receptor substrate 1; IRS-1
基因别名: G972R; HIRS-1; IRS-1; IRS1
UniProt ID: (Mouse) P35569
Entrez Gene ID: (Human) 3667, (Mouse) 16367
phosphotyrosine binding
transmembrane receptor protein tyrosine kinase adaptor activity
protein kinase C binding
receptor binding
insulin receptor binding
insulin-like growth factor receptor binding
protein binding
receptor signaling complex scaffold activity
signaling adaptor activity
SH2 domain binding
phosphatidylinositol 3-kinase binding
signal transducer activity
protein kinase binding
protein domain specific binding
signal transduction
positive regulation of cell proliferation
insulin receptor signaling pathway
positive regulation of signal transduction
positive regulation of glucose metabolic process
cytokine-mediated signaling pathway
positive regulation of fatty acid beta-oxidation
response to insulin
cellular response to insulin stimulus
glucose homeostasis
protein kinase B signaling
positive regulation of glycogen biosynthetic process
positive regulation of glucose import
negative regulation of insulin receptor signaling pathway
positive regulation of insulin receptor signaling pathway
negative regulation of insulin secretion
insulin-like growth factor receptor signaling pathway
positive regulation of protein kinase B signaling
cellular response to fatty acid
positive regulation of mesenchymal cell proliferation
regulation of gene expression
phosphatidylinositol 3-kinase signaling
lipid catabolic process
insulin secretion
positive regulation of cell migration
mammary gland development
response to caffeine
protein localization to nucleus
positive regulation of phosphorylation
response to peptide hormone
positive regulation of phosphatidylinositol 3-kinase activity
protein heterooligomerization
positive regulation of glucagon secretion
negative regulation of somatostatin secretion
positive regulation of glucose import in response to insulin stimulus
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