Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 11
Phospho-JAK1 (Tyr1034, Tyr1035) Antibody (44-422G) in ICC/IF
Immunofluorescence analysis of JAK1 (pY1034/1035) was done on 70% confluent log phase COLO 205 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with JAK1 (pY1034/1035) Rabbit polyclonal Antibody (Product # 44-422G) at 1:250 dilution in 1% BSA and incubated... View More
Product Details
44-422G
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
C. elegans,
Human,
Mouse,
Non-human primate,
Rabbit,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human JAK1 protein that contains tyrosines 1034 and 1035. The sequence is conserved in mouse and pig.
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product was formerly called "Phospho-JAK1 (Tyr1022, Tyr1023) Polyclonal Antibody" because Human Jak1 residues Tyr1034 and Tyr1035 have been historically referenced as Tyr1022 and Tyr1023.
Target Information
Janus kinase 1 (JAK1), is a protein tyrosine kinase that is involved in the interferon-alpha/beta and -gamma signal transduction pathways. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK1. JAK1 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. Upon ligand binding, JAK1 undergoes tyrosine phosphorylation and catalytic activation in an interdependent manner. Phosphorylation of tyrosine residues at positions 1022 and 1023 is believed to function in the activation of catalytic events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: EC 2.7.10.2; JAK-1; Janus kinase 1; kinase Jak1; Tyrosine-protein kinase JAK1
Gene Aliases: AA960307; BAP004; C130039L05Rik; JAK1; JAK1A; JAK1B; JTK3
UniProt ID: (Human) P23458
Entrez Gene ID: (Human) 3716, (Mouse) 16451
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
Ras guanyl-nucleotide exchange factor activity
receptor binding
growth hormone receptor binding
protein binding
ATP binding
protein phosphatase binding
ubiquitin protein ligase binding
CCR5 chemokine receptor binding
metal ion binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
MAPK cascade
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
cell migration
cell differentiation
intracellular signal transduction
peptidyl-tyrosine autophosphorylation
interleukin-2-mediated signaling pathway
regulation of cell proliferation
positive regulation of GTPase activity
innate immune response
response to antibiotic
interferon-gamma-mediated signaling pathway
regulation of interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
regulation of type I interferon-mediated signaling pathway
positive regulation of sprouting angiogenesis
enzyme linked receptor protein signaling pathway
phosphorylation
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
protein autophosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support