Invitrogen

Phospho-MSK1/MSK2 (Ser376/Ser360) Polyclonal Antibody

Name: Phospho-MSK1\/MSK2 (Ser376\/Ser360) Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-148474

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

Phospho-MSK1/MSK2 (Ser376/Ser360) Antibody in Western Blot (WB)

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Phospho-MSK1/MSK2 (Ser376/Ser360) Antibody (PA5-148474) in WB

Western Blot analysis of Phospho-MSK1/MSK2 (Ser376/Ser360) in HEK293 using Phospho-MSK1/MSK2 (Ser376/Ser360) polyclonal antibody (Product # PA5-148474).

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Immunogen image

Immunogen Image Phospho-MSK1/MSK2 (Ser376/Ser360) Polyclonal Antibody

产品信息

PA5-148474

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.2 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Phosphopeptide containing human MSK1 S376 site

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with trehalose

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_3707298

产品详细信息

Reconstitute at 0.2 mg/mL in sterile PBS.

靶标信息

MSK1 is a mitogen and stress activated protein kinase 1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK1 can be activated by ERK1/2 and SAPK2/p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG14 in response to mitogen and stress. Similar to RSK, MSK1 contains two kinase domains (N term and a C term). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C terminal kinase domain of MSK1 which is essential for the catalytic activity of the N terminal kinase domain. MSK2 plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 90 kDa ribosomal protein S6 kinase 4; 90 kDa ribosomal protein S6 kinase 5; mitogen- and stress-activated protein kinase 2; Nuclear mitogen- and stress-activated protein kinase 1; Nuclear mitogen- and stress-activated protein kinase 2; Ribosomal protein kinase B; ribosomal protein S6 kinase alpha 4; Ribosomal protein S6 kinase alpha-4; Ribosomal protein S6 kinase alpha-5; ribosomal protein S6 kinase, 90kDa, polypeptide 4; ribosomal protein S6 kinase, 90kDa, polypeptide 5; RSK-like protein kinase; RSKB; RSKL; S6K-alpha-4; S6K-alpha-5

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基因别名: MSK1; MSK2; MSPK1; RLPK; RPS6KA4; RPS6KA5; RSK-B; S6K-alpha-4

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UniProt ID: (Human) O75676, (Human) O75582

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Entrez Gene ID: (Human) 8986, (Human) 9252

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