Invitrogen

Phospho-Nrf2 (Ser40) Recombinant Rabbit Monoclonal Antibody (2Z5J6)

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问题与答案

问题与答案

问题与答案

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抗体检测数据 (6)

Phospho-Nrf2 (Ser40) Antibody in Immunocytochemistry (ICC/IF)

Phospho-Nrf2 (Ser40) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-Nrf2 (Ser40) Antibody (MA569906) in ICC/IF

Immunocytochemical analysis of Phospho-Nrf2 (Ser40) in Hep G2 cells. Samples were incubated with Phospho-Nrf2 (Ser40) recombinant monoclonal antibody (Product # MA569906) using a dilution of 1:100 (40x lens). Followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) using a dilution of 1:500 (Red). The cells were counterstained with alpha-Tubulin Mouse mAb using dilution of 1:400. Followed by inc... View More

Phospho-Nrf2 (Ser40) Antibody in Immunocytochemistry (ICC/IF)

Phospho-Nrf2 (Ser40) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-Nrf2 (Ser40) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-Nrf2 (Ser40) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-Nrf2 (Ser40) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-Nrf2 (Ser40) Antibody in Western Blot (WB)

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Phospho-Nrf2 (Ser40) Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of Phospho-Nrf2 (Ser40) in Hep G2 cells. Samples were incubated with Phospho-Nrf2 (Ser40) recombinant monoclonal antibody (Product # MA569906) using a dilution of 1:100 (40x lens). Followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) using a dilution of 1:500 (Red). The cells were counterstained with alpha-Tubulin Mouse mAb using dilution of 1:400. Followed by incubation with 488-conjugated Goat Anti-Mouse IgG (H+L) using a dilution of 1:500 (Green). DAPI was used for nuclear staining (Blue).

Phospho-Nrf2 (Ser40) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-Nrf2 (Ser40) on paraffin-embedded Mouse liver (treated with λpp) and Mouse liver (untreated) tissue. Samples were incubated with Phospho-Nrf2 (Ser40) recombinant monoclonal antibody (Product # MA569906) using a dilution of 1:100 (40x lens). Followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) using a dilution of 1:500 (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0).

Phospho-Nrf2 (Ser40) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-Nrf2 (Ser40) on paraffin-embedded Human tonsil tissue, Untreated (left) and lambda phosphatase-treated (right) tissue. Samples were incubated with Phospho-Nrf2 (Ser40) recombinant monoclonal antibody (Product # MA569906) using a dilution of 1:1,600 (40x lens). High pressure antigen retrieval performed with 0. 01 M Tris-EDTA Buffer (pH 9. 0).

Phospho-Nrf2 (Ser40) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-Nrf2 (Ser40) on paraffin-embedded Mouse liver tissue, Untreated (left) and lambda phosphatase-treated (right) tissue. Samples were incubated with Phospho-Nrf2 (Ser40) recombinant monoclonal antibody (Product # MA569906) using a dilution of 1:1,600 (40x lens). High pressure antigen retrieval performed with 0. 01 M Tris-EDTA Buffer (pH 9. 0).

Phospho-Nrf2 (Ser40) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-Nrf2 (Ser40) on paraffin-embedded Rat brain tissue, Untreated (left) and lambda phosphatase-treated (right) tissue. Samples were incubated with Phospho-Nrf2 (Ser40) recombinant monoclonal antibody (Product # MA569906) using a dilution of 1:1,600 (40x lens). High pressure antigen retrieval performed with 0. 01 M Tris-EDTA Buffer (pH 9. 0).

Phospho-Nrf2 (Ser40) Antibody in Western Blot (WB) — Western blot analysis of Phospho-Nrf2 (Ser40) in 293T cells. Samples were probed with Phospho-Nrf2 (Ser40) recombinant monoclonal antibody (Product # MA569906) at 1:10,000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 30 µg per lane. Blocking buffer: 3 % nonfat dry milk in TBST.

产品信息

MA569906

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000-1:10,000

-

免疫组化（石蜡） (IHC (P))

1:500-1:2,000

-

免疫细胞化学 (ICC/IF)

1:50-1:200

-

酶联免疫吸附实验 (ELISA)

1 µg/mL

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

2Z5J6

抗原

Synthetic peptide.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.82 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3., with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

Nfr1 (also designated TCF11), Nrf2 and Nfr3 are bZIP transcription factors that heterodimer with Maf proteins to bind MARE (Maf recognition elements) sequences. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: E4TF1-60; E4TF1A; NF-E2-like basic leucine zipper transcriptional activator; NF-E2-related factor 2; NFE2-related factor 2; NFT2; NRF2; NRF2A; nuclear factor erythroid 2-like 2; Nuclear factor erythroid 2-related factor 2; nuclear factor erythroid-derived 2-like 2; nuclear factor, erythroid 2 like 2; Nuclear factor, erythroid derived 2, like 2; unnamed protein product

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基因别名: AI194320; HEBP1; IMDDHH; NFE2L2; Nrf-2; NRF2

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UniProt ID: (Human) Q16236, (Mouse) Q60795, (Rat) O54968

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Entrez Gene ID: (Human) 4780, (Mouse) 18024, (Rat) 83619

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transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription cofactor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding protein domain specific binding ubiquitin protein ligase binding sequence-specific DNA binding RNA polymerase II sequence-specific DNA binding transcription factor binding molecular condensate scaffold activity RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II activating transcription factor binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding protein heterodimerization activity basic leucine zipper transcription factor

response to ischemia regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter inflammatory response response to oxidative stress response to xenobiotic stimulus gene expression proteasomal ubiquitin-independent protein catabolic process positive regulation of gene expression negative regulation of cardiac muscle cell apoptotic process positive regulation of neuron projection development protein ubiquitination positive regulation of blood coagulation endoplasmic reticulum unfolded protein response cellular response to oxidative stress response to endoplasmic reticulum stress PERK-mediated unfolded protein response cellular response to glucose starvation proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of blood vessel endothelial cell migration regulation of innate immune response cell redox homeostasis positive regulation of angiogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of embryonic development aflatoxin catabolic process positive regulation of glucose import cellular response to methionine response to caloric restriction cellular response to hydrogen peroxide cellular response to copper ion cellular response to tumor necrosis factor cellular response to hypoxia cellular response to xenobiotic stimulus cellular response to fluid shear stress cellular response to laminar fluid shear stress reactive oxygen species metabolic process negative regulation of ferroptosis integrated stress response signaling negative regulation of cellular response to hypoxia regulation of cellular response to oxidative stress negative regulation of hematopoietic stem cell differentiation negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway positive regulation of glutathione biosynthetic process positive regulation of ERAD pathway cellular response to angiotensin negative regulation of vascular associated smooth muscle cell migration positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process regulation of removal of superoxide radicals negative regulation of endothelial cell apoptotic process positive regulation of reactive oxygen species metabolic process transcription, DNA-templated transcription from RNA polymerase II promoter cellular response to drug positive regulation of transcription from RNA polymerase II promoter in response to stress positive regulation of transcription from RNA polymerase II promoter in response to oxidative stress negative regulation of cell death negative regulation of hydrogen peroxide-induced cell death