Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Phospho-PAK4/6/7 (Ser474) Recombinant Rabbit Monoclonal Antibody (1H7L3)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
图: 1 / 2
Phospho-PAK4/6/7 (Ser474) Antibody (702663) in ICC/IF
For immunofluorescence analysis, MCF-7 cells were fixed and permeabilized for detection of Phospho-PAK4 + PAK6 + PAK7 pSer474 using Anti- Phospho-PAK4 + PAK6 + PAK7 pSer474 Recombinant Rabbit Monoclonal Antibody (Product # 702663, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representativ... View More
产品信息
702663
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
1H7L3
抗原
Peptide corresponding to human Phospho-PAK4/PAK5/PAK6 (Ser474) (aa472-aa480)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
This antibody is predicted to react with Monkey, Horse, Rat
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
PAK4 is highly expressed in prostate, testis and colon. PAK4 interacts tightly with GTP-bound but not GDP-bound CDC42 and weakly with RAC. PAK4 phosphorylates and autophosphorylates and also activates the JNK pathway. PAK5, a member of the PAK family of protein kinases contains a CDC42/Rac1 interactive binding (CRIB) motif at the N-terminus and a Ste20-like kinase domain at the C-terminus. PAK5 preferentially binds to CDC42 in the presence of GTP and the CRIB motif is essential for this interaction. PAK6 protein cotranslocates into the nucleus with androgen receptor, which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development, in response to androgen. PAK6 was initially identified as an androgen receptor in a yeast two hybrid screen and was found to be highly expressed in testis and prostate tissues. Later experiments have shown it to be activated by MAP kinase 6 and p38 MAP kinase, suggesting that PAK6 may play a role in the cellular response to stress-related signals.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: p21 (RAC1) activated kinase 7; p21 protein (Cdc42/Rac)-activated kinase 4; p21 protein (Cdc42/Rac)-activated kinase 6; p21 protein (Cdc42/Rac)-activated kinase 7; p21(CDKN1A)-activated kinase 4; p21(CDKN1A)-activated kinase 6; p21(CDKN1A)-activated kinase 7; p21-activated kinase 4; p21-activated kinase 5; p21-activated kinase 6; p21-activated kinase 7; p21CDKN1A-activated kinase 7; PAK 4/5/6; PAK 4/6/7; PAK-4; PAK-5; PAK-6; PAK-7; PAK4/5/6; protein kinase PAK5; protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs; Serine/threonine-protein kinase PAK 4; Serine/threonine-protein kinase PAK 5; Serine/threonine-protein kinase PAK 6; serine/threonine-protein kinase PAK7
基因别名: KIAA1142; KIAA1264; PAK4; PAK5; PAK6; PAK7
UniProt ID: (Human) O96013, (Human) Q9P286, (Human) Q9NQU5
Entrez Gene ID: (Human) 10298, (Human) 57144, (Human) 56924
mitotic cell cycle
apoptotic process
movement of cell or subcellular component
cytoskeleton organization
signal transduction
cell proliferation
cell growth
cell migration
signal transduction by protein phosphorylation
dendritic spine development
cellular response to organic cyclic compound
cell-cell adhesion
learning
memory
locomotory behavior
negative regulation of extrinsic apoptotic signaling pathway
regulation of transcription, DNA-templated
