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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • PKC Pan Antibodies

          Invitrogen

          Phospho-PKC Pan (Thr497) Polyclonal Antibody

          高级验证
          查看其他7个PKC Pan抗体

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          Cite Phospho-PKC Pan (Thr497) Polyclonal Antibody

          • 抗体检测数据 (4)
          • 高级验证 (1)
          Phospho-PKC Pan (Thr497) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          Phospho-PKC Pan (Thr497) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          图: 1 / 5

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          Phospho-PKC Pan (Thr497) Antibody (PA5-36757) in IHC (P)

          Immunohistochemistry analysis of PKC Pan in paraffin-embedded human breast carcinoma tissue. Samples were incubated with PKC Pan polyclonal antibody (Product # PA5-36757). {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
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          Phospho-PKC Pan (Thr497) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-PKC Pan (Thr497) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-PKC Pan (Thr497) Antibody in Western Blot (WB)
          Phospho-PKC Pan (Thr497) Antibody in Western Blot (WB)
          Phospho-PKC Pan (Thr497) Antibody

          产品信息

          PA5-36757

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:500-1:1,000
          -

          免疫组化(石蜡) (IHC (P))

          1:50-1:200
          -
          产品规格

          种属反应

          Human, Mouse, Rat

          宿主/亚型

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          抗原

          Synthetic phosphopeptide derived from human PKC-pan around the phosphorylation site of Threonine 497

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          1 mg/mL

          纯化类型

          Antigen affinity chromatography

          保存液

          PBS, pH 7.2, with 50% glycerol

          内含物

          0.02% sodium azide

          保存条件

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2553707

          产品详细信息

          This antibody detects endogenous levels of PKC alpha, beta-I, beta-II, gamma, delta, epsilon, eta, zeta and theta isoforms only when phosphorylated at a residue homologous to threonine 497 of human PKC alpha.

          Purity is >95% by SDS-PAGE.

          靶标信息

          Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms ( alpha, betaI, betaII and gamma) and novel (n) PKC isoforms ( delta, epsilon, zeta, eta and theta). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and epsilon are independent of Ca2+.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          篇参考文献 (0)

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          引用该产品

          生物信息学

          蛋白别名: 14 - 3 - 3 - zeta isoform; aPKC-lambda/iota; atypical protein kinase C; Atypical protein kinase C-lambda/iota; nPKC-delta; nPKC-epsilon; nPKC-eta; nPKC-iota; nPKC-theta; nPKC-zeta; PKC theta; PKC zeta; PKC-A; PKC-alpha; PKC-B; PKC-beta; PKC-gamma; PKC-L; PRKC-lambda/iota; Protein kinase C alpha type; protein kinase C beta I; protein kinase C beta II; Protein kinase C beta type; protein kinase C beta-II; Protein kinase C delta type; protein kinase C delta variant IX; protein kinase C delta VIII; Protein kinase C epsilon type; Protein kinase C eta type; Protein kinase C gamma type; Protein kinase C iota type; Protein kinase C theta type; protein kinase C type I (gamma type); protein kinase C zeta subspecies; Protein kinase C zeta type; protein kinase C, beta 1; protein kinase C, beta 1 polypeptide; protein kinase C, delta; protein kinase C, delta IV; protein kinase C, delta V; protein kinase C, epsilon; protein kinase C, eta; protein kinase C, gamma; protein kinase C, iota; protein kinase C, lambda; protein kinase C, theta; protein kinase C, zeta; protein kinase c-alpha; protein kinase C-eta; protein kinase C[d]; Tyrosine-protein kinase PRKCD

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          基因别名: 14-3-3-zetaisoform; 2310021H13Rik; 5830406C15Rik; A130035A12Rik; A130082F03Rik; AI098070; AI385711; AI427505; AI875142; ALPS3; aPKClambda; aPKCzeta; AW494342; C80388; CVID9; D14Ertd420e; DXS1179E; MAY1; mKIAA4165; nPKC-delta; nPKC-epsilon; nPKC-eta; nPKC-iota; nPKC-theta; nPKC-zeta; PKC; PKC-0; PKC-Beta; PKC-gamma; PKC-L; PKC-theta; PKC-ZETA; PKC2; PKC[d]; PKC[e]; Pkca; PKCB; PKCC; PKCD; PKCdelta; PKCE; Pkcea; PKCepsilon; PKCG; PKCgamma; Pkch; PKCI; PKCL; PKClambda; Pkcq; PKCtheta; Pkcz; Prkc; Prkca; PRKCB; PRKCB1; PRKCB2; Prkcc; PRKCD; PRKCE; PRKCG; PRKCH; PRKCI; PRKCL; PRKCQ; PRKCT; PRKCZ; r14-3-3; R74924; R75156; RATPKCI; SCA14; zetaPKC

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          UniProt ID: (Human) P05771, (Human) Q05655, (Human) Q02156, (Human) P05129, (Human) P24723, (Human) P41743, (Human) Q04759, (Human) Q05513, (Mouse) P20444, (Mouse) P68404, (Rat) P68403, (Mouse) P28867, (Rat) P09215, (Mouse) P16054, (Rat) P09216, (Mouse) P63318, (Rat) P63319, (Mouse) P23298, (Rat) Q64617, (Mouse) Q62074, (Rat) F1M7Y5, (Mouse) Q02111, (Rat) P09217, (Mouse) Q02956

          查看更多 收起

          Entrez Gene ID: (Human) 5579, (Human) 5580, (Human) 5581, (Human) 5582, (Human) 5583, (Human) 5584, (Human) 5588, (Human) 5590, (Rat) 24680, (Mouse) 18750, (Mouse) 18751, (Rat) 25023, (Mouse) 18753, (Rat) 170538, (Mouse) 18754, (Rat) 29340, (Mouse) 18752, (Rat) 24681, (Mouse) 18755, (Rat) 81749, (Mouse) 18759, (Rat) 84006, (Rat) 85420, (Mouse) 18761, (Rat) 25522, (Mouse) 18762

          查看更多 收起

          功能
          chromatin binding protein serine/threonine kinase activity protein kinase C activity calcium-dependent protein kinase C activity protein kinase C binding calcium channel regulator activity protein binding ATP binding zinc ion binding ligand-dependent nuclear receptor transcription coactivator activity histone kinase activity (H3-T6 specific) histone binding androgen receptor binding protein kinase activity calcium-independent protein kinase C activity non-membrane spanning protein tyrosine kinase activity enzyme activator activity enzyme binding kinase binding protein kinase binding insulin receptor substrate binding metal ion binding actin monomer binding signal transducer activity receptor activator activity ethanol binding 14-3-3 protein binding protein serine/threonine/tyrosine kinase activity Ral GTPase binding phospholipid binding protein domain specific binding potassium channel regulator activity phospholipase binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups TIR domain binding receptor binding SH3 domain binding molecular_function non-receptor serine/threonine protein kinase protein modifying enzyme
          参与通路
          response to hypoxia adaptive immune response transcription, DNA-templated regulation of transcription from RNA polymerase II promoter protein phosphorylation calcium ion transport cellular calcium ion homeostasis apoptotic process mitotic nuclear envelope disassembly signal transduction negative regulation of glucose transport peptidyl-serine phosphorylation platelet activation positive regulation of vascular endothelial growth factor receptor signaling pathway histone H3-T6 phosphorylation intracellular signal transduction B cell activation lipoprotein transport positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of angiogenesis negative regulation of insulin receptor signaling pathway B cell receptor signaling pathway positive regulation of B cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity cellular response to carbohydrate stimulus stimulatory C-type lectin receptor signaling pathway cellular component disassembly involved in execution phase of apoptosis cell cycle activation of phospholipase C activity intrinsic apoptotic signaling pathway in response to oxidative stress regulation of receptor activity immunoglobulin mediated immune response histone phosphorylation peptidyl-threonine phosphorylation peptidyl-tyrosine phosphorylation termination of signal transduction negative regulation of actin filament polymerization positive regulation of endodeoxyribonuclease activity negative regulation of protein binding activation of protein kinase activity interleukin-10 production interleukin-12 production positive regulation of superoxide anion generation regulation of actin cytoskeleton organization negative regulation of glial cell apoptotic process positive regulation of protein dephosphorylation Fc-gamma receptor signaling pathway involved in phagocytosis B cell proliferation neutrophil activation positive regulation of protein import into nucleus defense response to bacterium negative regulation of MAP kinase activity regulation of mRNA stability negative regulation of inflammatory response negative regulation of peptidyl-tyrosine phosphorylation protein stabilization negative regulation of filopodium assembly cell chemotaxis interferon-gamma-mediated signaling pathway cellular response to hydrogen peroxide cellular response to hydroperoxide negative regulation of platelet aggregation cellular senescence positive regulation of phospholipid scramblase activity cellular response to angiotensin positive regulation of ceramide biosynthetic process positive regulation of glucosylceramide catabolic process positive regulation of sphingomyelin catabolic process positive regulation of response to DNA damage stimulus positive regulation of apoptotic signaling pathway macrophage activation involved in immune response cell adhesion positive regulation of epithelial cell migration positive regulation of fibroblast migration positive regulation of cell-substrate adhesion positive regulation of actin filament polymerization negative regulation of protein ubiquitination lipopolysaccharide-mediated signaling pathway positive regulation of insulin secretion positive regulation of synaptic transmission, GABAergic positive regulation of cytokinesis locomotory exploration behavior TRAM-dependent toll-like receptor 4 signaling pathway response to morphine positive regulation of MAPK cascade regulation of peptidyl-tyrosine phosphorylation positive regulation of lipid catabolic process release of sequestered calcium ion into cytosol regulation of release of sequestered calcium ion into cytosol cell division regulation of insulin secretion involved in cellular response to glucose stimulus positive regulation of mucus secretion cellular response to ethanol cellular response to prostaglandin E stimulus cellular response to hypoxia positive regulation of wound healing positive regulation of receptor activity negative regulation of sodium ion transmembrane transporter activity positive regulation of cellular glucuronidation chemical synaptic transmission learning or memory chemosensory behavior phosphorylation regulation of response to food positive regulation of mismatch repair negative regulation of protein catabolic process regulation of circadian rhythm negative regulation of neuron apoptotic process protein autophosphorylation response to pain rhythmic process regulation of phagocytosis innervation negative regulation of proteasomal protein catabolic process positive regulation of macrophage derived foam cell differentiation positive regulation of keratinocyte differentiation positive regulation of glial cell proliferation protein kinase C signaling regulation of bicellular tight junction assembly protein targeting to membrane cytoskeleton organization actin filament organization positive regulation of neuron projection development vesicle-mediated transport cell migration cellular response to insulin stimulus establishment of apical/basal cell polarity eye photoreceptor cell development negative regulation of apoptotic process establishment or maintenance of epithelial cell apical/basal polarity cell-cell junction organization positive regulation of glucose import secretion Golgi vesicle budding membrane organization response to interleukin-1 bicellular tight junction assembly positive regulation of establishment of protein localization to plasma membrane positive regulation of endothelial cell apoptotic process regulation of cell growth regulation of transcription, DNA-templated membrane protein ectodomain proteolysis inflammatory response axon guidance aging response to heat response to glucose regulation of G2/M transition of mitotic cell cycle response to organic cyclic compound regulation of vasoconstriction positive regulation of telomere maintenance via telomerase positive regulation of interleukin-17 production positive regulation of interleukin-4 production response to insulin Fc-epsilon receptor signaling pathway positive regulation of T cell proliferation tissue regeneration positive regulation of NF-kappaB import into nucleus positive regulation of interleukin-2 biosynthetic process respiratory burst positive regulation of protein secretion T cell receptor signaling pathway positive regulation of T cell activation positive regulation of filopodium assembly positive regulation of stress fiber assembly positive regulation of telomerase activity negative regulation of T cell apoptotic process regulation of platelet aggregation positive regulation of telomere capping positive regulation of T-helper 17 type immune response positive regulation of T-helper 2 cell activation microtubule cytoskeleton organization positive regulation of cell-matrix adhesion transforming growth factor beta receptor signaling pathway long-term memory positive regulation of cell proliferation insulin receptor signaling pathway establishment of cell polarity negative regulation of protein complex assembly actin cytoskeleton reorganization activation of phospholipase D activity activation of protein kinase B activity positive regulation of T-helper 2 cell differentiation positive regulation of insulin receptor signaling pathway vesicle transport along microtubule protein heterooligomerization negative regulation of hydrolase activity membrane depolarization membrane hyperpolarization long-term synaptic potentiation positive regulation of ERK1 and ERK2 cascade protein localization to plasma membrane neuron projection extension positive regulation of excitatory postsynaptic potential positive regulation of T-helper 2 cell cytokine production positive regulation of interleukin-5 secretion positive regulation of interleukin-13 secretion positive regulation of interleukin-10 secretion inactivation of MAPK activity response to reactive oxygen species angiogenesis negative regulation of protein phosphorylation positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation regulation of the force of heart contraction chondrocyte differentiation desmosome assembly negative regulation of protein kinase activity regulation of muscle contraction negative regulation of cell proliferation response to mechanical stimulus response to toxic substance positive regulation of endothelial cell migration positive regulation of cardiac muscle hypertrophy negative regulation of translation central nervous system neuron axonogenesis positive regulation of cell migration neutrophil chemotaxis positive regulation of lipopolysaccharide-mediated signaling pathway response to estradiol peptidyl-serine autophosphorylation response to peptide hormone positive regulation of blood vessel endothelial cell migration establishment of protein localization response to ethanol positive regulation of macrophage differentiation positive regulation of cell adhesion negative regulation of heart contraction positive regulation of exocytosis positive regulation of mitotic cell cycle negative regulation of glucose import response to antibiotic regulation of receptor-mediated endocytosis positive regulation of smooth muscle cell proliferation positive regulation of inflammatory response induction of positive chemotaxis response to corticosterone positive regulation of synapse assembly intrinsic apoptotic signaling pathway positive regulation of dense core granule biogenesis immune system process regulation of dopamine secretion chromatin modification regulation of growth response to drug dibenzo-p-dioxin metabolic process response to vitamin D positive regulation of odontogenesis of dentin-containing tooth response to oxidative stress aspartate transport collagen metabolic process cellular response to oxidative stress regulation of phosphorylation positive regulation of apoptotic process positive regulation of MAP kinase activity response to organonitrogen compound cellular response to glucose starvation response to hydrogen peroxide positive regulation of catalytic activity response to amino acid negative regulation of mitochondrial membrane potential regulation of lipid metabolic process cellular response to platelet-derived growth factor stimulus negative regulation of release of sequestered calcium ion into cytosol negative regulation of mitochondrial calcium ion concentration cell differentiation multicellular organism development cellular protein localization neuron differentiation cell surface receptor signaling pathway positive regulation of synaptic transmission positive regulation of protein transport
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          Host server : magellan-search-blue-555ccb4fbd-6kdvm:80/100.66.79.196:80.
          git-commit: 75f0c7d4cb44686da2af39b21f2e6446a20282e2
          git-url: https://github.com/thermofisher/magellan-search
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