Invitrogen

Phospho-PKC epsilon (Ser729) Polyclonal Antibody

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问题与答案

问题与答案

Phospho-PKC epsilon (Ser729) Antibody in Western Blot (WB)

Phospho-PKC epsilon (Ser729) Antibody in Western Blot (WB)

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Phospho-PKC epsilon (Ser729) Antibody (44-977G) in WB

Peptide Competition and Phosphatase Treatment: Lysates prepared from K562 cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda.

Phospho-PKC epsilon (Ser729) Antibody in Western Blot (WB)

Phospho-PKC epsilon (Ser729) Antibody in Western Blot (WB)

Phospho-PKC epsilon (Ser729) Antibody in Western Blot (WB)

Phospho-PKC epsilon (Ser729) Antibody

Product Image Gallery

Phospho-PKC epsilon (Ser729) Antibody in Western Blot (WB) — Peptide Competition and Phosphatase Treatment: Lysates prepared from K562 cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda.

Phospho-PKC epsilon (Ser729) Antibody in Western Blot (WB) — Published figure using Phospho-PKC epsilon (Ser729) polyclonal antibody (Product # 44-977G) in Western Blot

Phospho-PKC epsilon (Ser729) Antibody in Western Blot (WB) — Published figure using Phospho-PKC epsilon (Ser729) polyclonal antibody (Product # 44-977G) in Western Blot

Phospho-PKC epsilon (Ser729) Antibody — Figure 3 PKCepsilon phosphorylation of ATF2 on Thr52 negatively regulates its mitochondrial localization. (See also Figure S3 ) Coverslip-grown SCC9 cells were immunofluorescently stained with indicated antibodies after: A) overnight treatment with 1 or 5 muM PKCepsilon translocation inhibitor (PKCepsilon-i); B) overnight treatment with 20 nM scrambled control (siSC) or PKCepsilon-targeted (siPKCepsilon) siRNA; C) transfection with HIS-tagged constitutively active PKCepsilon (caPKCepsilon, red) and treatment with 5 muM etoposide (ETO, overnight). D) SCC9 cells treated overnight with ETO or 5 muM PKCepsilon translocation inhibitor (PKCepsilon-i) were lysed and subject to immunoblot analysis with indicated antibodies. E) In vitro PKCepsilon kinase assay with Millipore control peptide (CTL), purified GST-ATF2 50-100aa WT (WT), GST-ATF2 50-100aa WT + PKCepsilon catalytic inhibitor, Go6850 (WT+Go) (3) or GST-ATF2 50-100aa T52A (52A) as substrate. *: non-specific bands. Coomassie-stained gel (left) and autoradiograph (right) are displayed as indicated. F) Coverslip-grown SCC9 cells were transfected with HA-tagged wild-type ATF2 (WT), ATF2 T52A or ATF2 T52E , and subsequently immunofluorescently stained with indicated antibodies. G) SCC9 control or ATF2 knocked-down cells (shATF2) were transfected with Jun2 promoter-luciferase construct and either empty vector (EV) or caPKCepsilon were assayed for luciferase activity before and after overnight ETO or Go6850 (2 muM) treatment.*: p = {TM}

Immunogen image

Phospho-PKC epsilon (Ser729) Polyclonal Antibody

Immunogen Image Phospho-PKC epsilon (Ser729) Polyclonal Antibody

产品信息

44-977G

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化（冰冻） (IHC (F))

-

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Virus

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PKCe that contains serine 729. The sequence is conserved in mouse and rat.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

The PKC family of serine/threonine kinases, including PRKCE (PKC epsilon), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: MGC125656; MGC125657; nPKC-epsilon; PKC e; Protein kinase C epsilon type; protein kinase C, epsilon

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基因别名: 5830406C15Rik; nPKC-epsilon; PKC[e]; PKCE; Pkcea; PKCepsilon; PRKCE; R75156

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UniProt ID: (Human) Q02156, (Mouse) P16054

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Entrez Gene ID: (Human) 5581, (Mouse) 18754

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actin monomer binding protein serine/threonine kinase activity protein kinase C activity calcium-independent protein kinase C activity signal transducer activity protein binding ATP binding enzyme activator activity enzyme binding receptor activator activity ethanol binding metal ion binding 14-3-3 protein binding nucleotide binding protein kinase activity receptor binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups SH3 domain binding protein kinase binding non-receptor serine/threonine protein kinase protein modifying enzyme

macrophage activation involved in immune response protein phosphorylation apoptotic process cell cycle cell adhesion signal transduction activation of phospholipase C activity positive regulation of epithelial cell migration positive regulation of fibroblast migration positive regulation of cell-substrate adhesion peptidyl-serine phosphorylation platelet activation positive regulation of actin filament polymerization negative regulation of protein ubiquitination lipopolysaccharide-mediated signaling pathway positive regulation of insulin secretion positive regulation of synaptic transmission, GABAergic positive regulation of cytokinesis intracellular signal transduction locomotory exploration behavior TRAM-dependent toll-like receptor 4 signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis positive regulation of I-kappaB kinase/NF-kappaB signaling response to morphine positive regulation of MAPK cascade regulation of peptidyl-tyrosine phosphorylation positive regulation of lipid catabolic process release of sequestered calcium ion into cytosol regulation of release of sequestered calcium ion into cytosol cell division regulation of insulin secretion involved in cellular response to glucose stimulus positive regulation of mucus secretion cellular response to ethanol cellular response to prostaglandin E stimulus cellular response to hypoxia positive regulation of wound healing positive regulation of receptor activity negative regulation of sodium ion transmembrane transporter activity positive regulation of cellular glucuronidation immune system process chemosensory behavior negative regulation of mitochondrial membrane potential phosphorylation regulation of lipid metabolic process cellular response to platelet-derived growth factor stimulus negative regulation of apoptotic process positive regulation of catalytic activity negative regulation of release of sequestered calcium ion into cytosol negative regulation of mitochondrial calcium ion concentration