Invitrogen

Phospho-PKC theta (Thr538) Recombinant Rabbit Monoclonal Antibody (F4H4L1)

Name: Phospho-PKC theta (Thr538) Recombinant Rabbit Monoclonal Antibody (F4H4L1)
Brand: Invitrogen
SKU: 700043

高级验证

3个已发表图片

5 篇参考文献

查看其他23个PKC theta抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Phospho-PKC theta (Thr538) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Phospho-PKC theta (Thr538) Antibody (700043) in IHC (P)

Immunohistochemistry analysis of Phospho-PKC theta pThr538 in formalin-fixed, paraffin-embedded human breast carcimona tissue using a Phospho-PKC theta pThr538 monoclonal antibody (Product # 700043) at a dilution of 5 µg/mL. Staining was visualized using DAB. Images were taken at a magnification of 20x. Results show cytoplasmic staining in tumor cells.

Phospho-PKC theta (Thr538) Antibody in Western Blot (WB)

Phospho-PKC theta (Thr538) Antibody in Flow Cytometry (Flow)

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Immunogen image

Immunogen Image Phospho-PKC theta (Thr538) Recombinant Rabbit Monoclonal Antibody (F4H4L1)

产品信息

700043

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2-4 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

4-6 µg/mL

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流式细胞分析 (Flow)

1:100-1:500

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

F4H4L1

抗原

A peptide corresponding to amino acids 531-539 of Q04759.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2532274

产品详细信息

This antibody is predicted to react with bovine, chimpanzee, mouse, rat and Xenopus based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Protein Kinase C (PKC) isoforms are serine/threonine kinases involved in signal transduction pathways that govern a wide range of physiological processes including differentiation, proliferation, gene expression, brain function, membrane transport and the organization of cytoskeletal and extracellular matrix proteins. Increasing evidence from studies using in vitro and in vivo systems points to PKC as a key regulator of critical cell cycle transitions, including cell cycle entry and exit and the G1 and G2 checkpoints. PKC-mediated control of these transitions can be negative or positive, depending on the timing of PKC activation during the cell cycle and on the specific PKC isozymes involve. There have been at least 12 different PKC isoforms identified in humans to date, including alpha, beta I, beta II, gamma, delta, epsilon, zeta, eta, theta, iota, lambda, and mu. The different PKC isoforms vary in structure, subcellular localization, tissue distribution and response to extracellular signals. PKC beta II has been shown to be expressed, in part, in cardiac muscle, intestinal epithelium, kidney and pancreas. PKC beta II has been shown to be involved in cell cycle progression and cancer proliferation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。