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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • PTEN Antibodies

          Rockland

          Phospho-PTEN-P1 (Ser29, Thr32) Polyclonal Antibody

          查看其他23个PTEN抗体

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          Cite Phospho-PTEN-P1 (Ser29, Thr32) Polyclonal Antibody

          Phospho-PTEN-P1 (Ser29, Thr32) Polyclonal Antibody

          产品信息

          600-401-859

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:500- 1:2,000
          -

          酶联免疫吸附实验 (ELISA)

          1:20,000-1:80,000
          -

          免疫沉淀 (IP)

          1:500-1:2,000
          -
          产品规格

          种属反应

          Bovine, Dog, Chimpanzee, Human, Mouse, Rhesus monkey, Rat

          宿主/亚型

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          抗原

          This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 223-237 of human PTEN protein.
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          0.87 mg/mL

          纯化类型

          Affinity chromatography

          保存液

          0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

          内含物

          0.01% sodium azide

          保存条件

          -20°C, Avoid Freeze/Thaw Cycles

          运输条件

          Ambient (domestic); Wet ice (international)

          产品详细信息

          Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

          This affinity-purified antibody is directed against the phosphorylated form of human PTEN protein at the pS29 and pT32 residues. Reactivity occurs against human PTEN pS29/pT32 protein and the antibody is specific for the phosphorylated form of the proteins. Approximately 3% reactivity is observed against the non-phosphorylated form of the immunizing peptide. A BLAST analysis was used to suggest cross reactivity with PTEN proteins from mouse and dog sources based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

          靶标信息

          PTEN was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The PTEN gene is a phosphatidylinositol-3, 4, 5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. PTEN negatively regulates intracellular levels of phosphatidylinositol-3, 4, 5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. PTEN plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Also, PTEN may be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form of PTEN induces less tumor suppressive ability.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          篇参考文献 (0)

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          引用该产品

          生物信息学

          蛋白别名: homolog of human mutated in multiple advanced cancers; Inositol polyphosphate 3-phosphatase; mitochondrial phosphatase and tensin protein alpha; mitochondrial PTENalpha; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; Mutated in multiple advanced cancers 1; Phosphatase and tensin homolog; phosphatase and tensin homolog (mutated in multiple advanced cancers 1); phosphatase and tensin homolog deleted on chromosome ten; phosphatase and tensin-like protein; phosphatidylinositol 3; Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; phosphatidylinositol-3; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; protein tyrosine phosphatase and tensin-like protein; pten tumor suppressor

          查看更多 收起

          基因别名: 10q23del; 2310035O07Rik; A130070J02Rik; AI463227; B430203M17Rik; BZS; CWS1; DEC; GLM2; MHAM; Mmac; MMAC1; PTEN; PTEN1; rCG_47874; TEP1

          查看更多 收起

          UniProt ID: (Dog) P60483, (Human) P60484, (Rat) O54857, (Mouse) O08586

          查看更多 收起

          Entrez Gene ID: (Dog) 403832, (Human) 5728, (Bovine) 540786, (Rhesus monkey) 705121, (Rat) 50557, (Mouse) 19211

          查看更多 收起

          功能
          phosphatidylinositol-3-phosphatase activity protein serine/threonine phosphatase activity protein tyrosine phosphatase activity protein tyrosine/serine/threonine phosphatase activity anaphase-promoting complex binding phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity protein kinase binding PDZ domain binding identical protein binding inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity ubiquitin-specific protease binding magnesium ion binding phosphoprotein phosphatase activity platelet-derived growth factor receptor binding protein binding lipid binding enzyme binding hydrolase activity phosphatase activity protein phosphatase
          参与通路
          angiogenesis regulation of B cell apoptotic process protein dephosphorylation apoptotic process neuron-neuron synaptic transmission central nervous system development heart development learning or memory positive regulation of cell proliferation negative regulation of cell proliferation negative regulation of epithelial to mesenchymal transition regulation of neuron projection development cell migration dentate gyrus development central nervous system neuron axonogenesis negative regulation of cell migration adult behavior negative regulation of myelination regulation of protein stability regulation of synaptic transmission, GABAergic central nervous system myelin maintenance regulation of myeloid cell apoptotic process negative regulation of peptidyl-serine phosphorylation multicellular organismal response to stress social behavior peptidyl-tyrosine dephosphorylation maternal behavior negative regulation of apoptotic process protein kinase B signaling endothelial cell migration locomotor rhythm negative regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of cell size inositol phosphate dephosphorylation phosphatidylinositol dephosphorylation negative regulation of axon regeneration cardiac muscle tissue development forebrain morphogenesis negative regulation of epithelial cell proliferation negative regulation of axonogenesis protein stabilization positive regulation of sequence-specific DNA binding transcription factor activity negative regulation of keratinocyte migration negative regulation of focal adhesion assembly negative regulation of protein kinase B signaling rhythmic synaptic transmission negative regulation of cardiac muscle cell proliferation canonical Wnt signaling pathway synapse maturation prepulse inhibition male mating behavior long-term synaptic potentiation prostate gland growth dendritic spine morphogenesis negative regulation of dendritic spine morphogenesis negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade cellular response to electrical stimulus cellular response to hypoxia negative regulation of ribosome biogenesis negative regulation of cell aging negative regulation of excitatory postsynaptic potential presynaptic membrane assembly postsynaptic density assembly negative regulation of wound healing, spreading of epidermal cells positive regulation of TRAIL-activated apoptotic signaling pathway positive regulation of ubiquitin protein ligase activity negative regulation of vascular smooth muscle cell proliferation positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of G1/S transition of mitotic cell cycle positive regulation of excitatory postsynaptic potential negative regulation of synaptic vesicle clustering regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of protein phosphorylation phosphatidylinositol biosynthetic process synapse assembly aging response to nutrient memory locomotory behavior cell proliferation response to glucose response to zinc ion negative regulation of phosphatidylinositol 3-kinase signaling negative regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle response to estradiol regulation of cellular component size response to ATP response to drug inositol phosphate metabolic process response to ethanol negative regulation of organ growth response to arsenic-containing substance platelet-derived growth factor receptor signaling pathway phosphatidylinositol-mediated signaling regulation of axon regeneration brain morphogenesis negative regulation of phagocytosis T cell receptor signaling pathway long term synaptic depression response to organic substance response to inorganic substance response to organic cyclic compound dephosphorylation neuron projection development cellular response to decreased oxygen levels positive regulation of apoptotic process regulation of cell cycle positive regulation of apoptotic signaling pathway lipid metabolic process nervous system development
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