Invitrogen

Phospho-PYGL (Ser15) Polyclonal Antibody

Phospho-PYGL (Ser15) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Phospho-PYGL (Ser15) Antibody (PA5-114628) in IHC (P)

Immunohistochemistry analysis of Phospho-PYGL (Ser15) in rat ovarian tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114628) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Phospho-PYGL (Ser15) Antibody in Western Blot (WB)

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产品信息

PA5-114628

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:2,000

- -

免疫组化（石蜡） (IHC (P))

1:50-1:200

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human PYGL(Accession P06737), corresponding to amino acid residues around phosphorylated Ser15.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

sequential chromatography

保存液

PBS with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2899264

产品详细信息

Antibody detects endogenous levels of PYGL only when phosphorylated at Ser15.

靶标信息

This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。