Invitrogen

Phospho-Progesterone Receptor (Ser190) Polyclonal Antibody

Phospho-Progesterone Receptor (Ser190) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-Progesterone Receptor (Ser190) Antibody (PA5-114626) in ICC/IF

Immunocytochemistry analysis of Phospho-Progesterone Receptor (Ser190) in HeLa cells (heat shock treatment). Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114626). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), Alex... View More

Phospho-Progesterone Receptor (Ser190) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-Progesterone Receptor (Ser190) Antibody in Western Blot (WB)

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产品信息

PA5-114626

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human Progesterone Receptor around the phosphorylation site of Ser190.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

sequential chromatography

保存液

PBS with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2899262

产品详细信息

Antibody detects endogenous levels of Progesterone Receptor only when phosphorylated at Ser190.

靶标信息

The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.

仅用于科研。不用于诊断过程。未经明确授权不得转售。