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Invitrogen
Phospho-ROCK1 (Ser282) Polyclonal Antibody
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Phospho-ROCK1 (Ser282) Antibody (PA5-105055) in IHC (P)
Immunohistochemistry analysis of Phospho-ROCK1 (Ser282) in mouse colorectal tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-ROCK1 (Ser282) polyclonal antibody (Product # PA5-105055) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
产品信息
PA5-105055
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human ROCK1(Accession Q13464), corresponding to amino acid residues around phosphorylated Ser282.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
sequential chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of ROCK1 only when phosphorylated at Ser282.
靶标信息
ROCK1 is a serine/threonine kinase that regulates cell differentiation and migration. The Rho/ROCK pathway is involved in the progression of various human cancers. ROCK1 has been found to be a new Caspase-3 substrate. ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain that is located within a pleckstrin-homology region. They are joined by a variable region that contains the Rho-binding domain. During apoptosis, ROCK-I is cleaved by caspase-3 at a conserved DETD1113/G sequence and its carboxy-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3-mediated cleavage and activation of ROCK I induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: liver regeneration-related protein LRRG199; MGC131603; MGC43611; p150 RhoA-binding kinase ROK beta; p160 ROCK-1; p160-ROCK; p160ROCK; PRO0435; Renal carcinoma antigen NY-REN-35; Rho kinase; Rho-associated coiled-coil forming kinase 1; Rho-associated kinase beta; Rho-associated protein kinase 1; Rho-associated, coiled-coil containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I
基因别名: 1110055K06Rik; P160ROCK; ROCK-I; ROCK1
UniProt ID: (Human) Q13464, (Mouse) P70335
Entrez Gene ID: (Human) 6093, (Mouse) 19877, (Rat) 81762
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
GTP-Rho binding
metal ion binding
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Rho GTPase binding
non-receptor serine/threonine protein kinase
apical constriction
protein phosphorylation
cellular component disassembly involved in execution phase of apoptosis
smooth muscle contraction
leukocyte cell-cell adhesion
signal transduction
I-kappaB kinase/NF-kappaB signaling
Rho protein signal transduction
negative regulation of angiogenesis
membrane to membrane docking
actin cytoskeleton organization
regulation of cell adhesion
cortical actin cytoskeleton organization
bleb assembly
negative regulation of protein binding
regulation of actin cytoskeleton organization
negative regulation of myosin-light-chain-phosphatase activity
negative regulation of neuron apoptotic process
regulation of keratinocyte differentiation
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
leukocyte migration
leukocyte tethering or rolling
myoblast migration
regulation of stress fiber assembly
regulation of focal adhesion assembly
positive regulation of focal adhesion assembly
establishment of protein localization to plasma membrane
regulation of establishment of endothelial barrier
negative regulation of bicellular tight junction assembly
regulation of establishment of cell polarity
regulation of cell motility
apoptotic process
cytoskeleton organization
phosphorylation
regulation of actin filament-based process
intracellular signal transduction
regulation of cytoskeleton organization
