Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 5
Phospho-S6 (Ser235, Ser236) Antibody (740057MP647) in IHC (P)
Immunohistochemical analysis of Phospho-S6 was performed using formalin-fixed paraffin-embedded human breast cancer tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - Low pH (10X) (Product # 00-4955-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 m... View More
产品信息
740057MP647
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
cupk43k
偶联物
激发/发射光谱
658/675 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A/G
保存液
proprietary buffer, pH 6.3-7.3
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
RRID
产品详细信息
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: 40S ribosomal protein S6; OTTHUMP00000021121; OTTHUMP00000021122; Phosphoprotein NP33; RP11-513M16.6; RS6; Small ribosomal subunit protein eS6
基因别名: OK/SW-cl.2; RPS6; S6
UniProt ID: (Human) P62753
Entrez Gene ID: (Human) 6194
G1/S transition of mitotic cell cycle
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
placenta development
T cell proliferation involved in immune response
rRNA processing
translation
translational initiation
SRP-dependent cotranslational protein targeting to membrane
activation-induced cell death of T cells
mitotic nuclear division
mitotic cell cycle checkpoint
gastrulation
viral transcription
oogenesis stage
TOR signaling
T cell differentiation in thymus
ribosomal small subunit biogenesis
glucose homeostasis
positive regulation of apoptotic process
negative regulation of apoptotic process
erythrocyte development
