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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • SRC Antibodies

          Invitrogen

          Phospho-SRC (Tyr419) Polyclonal Antibody

          121 篇参考文献
          查看其他66个SRC抗体

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          说明书
          实验方案
          问题与答案
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          Cite Phospho-SRC (Tyr419) Polyclonal Antibody

          • 抗体检测数据 (2)
          Phospho-SRC (Tyr419) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          Phospho-SRC (Tyr419) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          图: 1 / 2

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          Phospho-SRC (Tyr419) Antibody (44-660G) in IHC (P)

          Immunohistochemistry analysis of SRC showing staining in the cytoplasm and membrane of paraffin-embedded human colon carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room te... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
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          查看产品

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          Phospho-SRC (Tyr419) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-SRC (Tyr419) Antibody in Western Blot (WB)
          Phospho-SRC (Tyr419) Polyclonal Antibody

          产品信息

          44-660G

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:1,000
          查看61篇已发表文章 61篇已发表文章

          免疫组化 (IHC)

          -
          查看16篇已发表文章 16篇已发表文章

          免疫组化(石蜡) (IHC (P))

          1:10-1:100
          -

          免疫组化(冰冻) (IHC (F))

          -
          查看1篇已发表文章 1篇已发表文章

          免疫细胞化学 (ICC/IF)

          -
          查看16篇已发表文章 16篇已发表文章

          酶联免疫吸附实验 (ELISA)

          -
          查看1篇已发表文章 1篇已发表文章

          免疫沉淀 (IP)

          -
          查看10篇已发表文章 10篇已发表文章

          亲和纯化 (AP)

          -
          查看7篇已发表文章 7篇已发表文章

          其他PubMed (Misc)

          -
          查看9篇已发表文章 9篇已发表文章
          产品规格

          种属反应

          Chicken, Human, Mouse

          已发表种属

          Bovine, C. elegans, Dog, Fruit fly, Human, Mouse, Pig, Rat, Tag, Xenopus, Zebrafish

          宿主/亚型

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          抗原

          The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Src that contains tyrosine 419. The sequence is conserved in mouse, rat, chicken and frog.
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          纯化类型

          Affinity chromatography

          保存液

          Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

          内含物

          0.05% sodium azide

          保存条件

          -20°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533714

          靶标信息

          SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in the regulation of embryonic development and cell growth. SRC is involved in the regulation of growth and differentiation of eukaryotic cells. SRC associates with the cytoplasmic region of various cell surface receptors to facilitate signal transduction, and is also involved in cytoskeletal organization, cell cycle regulation, and apoptosis. In humans, the gene encoding SRC is present on chromosome 20. Activity of SRC is regulated by two tyrosine residues (Tyr 416 and Tyr527), and phosphorylation of these two sites have opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates enzyme activity while the phosphorylation of Tyr527 decreases enzyme activity. Mutations in the SRC gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same SRC protein have been found thus far.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: c-src protein, C terminus; EC 2.7.10.2; neuronal proto-oncogene tyrosine-protein kinase Src; non-tyrosine protein kinase; ORF; OTTHUMP00000174476; OTTHUMP00000174477; OTTHUMP00000174478; p60-Src; pp60c-scr; pp60c-src; pp60src; Proto-oncogene c-Src; Proto-oncogene tyrosine-protein kinase Src; protooncogene SRC, Rous sarcoma; RP5-823N20.1; src downstream region; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; Unknown; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma viral oncogene

          查看更多 收起

          基因别名: ASV; AW259666; c-SRC; p60-Src; PP60C-SCR; pp60c-src; SDR; SRC; SRC1; THC6

          查看更多 收起

          UniProt ID: (Chicken) P00523, (Human) P12931, (Mouse) P05480

          查看更多 收起

          Entrez Gene ID: (Chicken) 396442, (Human) 6714, (Mouse) 20779

          查看更多 收起

          功能
          protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor binding protein binding ATP binding protein phosphatase binding heme binding growth factor receptor binding connexin binding protein kinase activity SH3/SH2 adaptor activity protein kinase C binding insulin receptor binding integrin binding protein C-terminus binding kinase activity enzyme binding kinase binding estrogen receptor binding SH2 domain binding ion channel binding ephrin receptor binding phosphoprotein binding hormone receptor binding scaffold protein binding cadherin binding involved in cell-cell adhesion nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding protein domain specific binding protein complex binding cell adhesion molecule binding transmembrane signal receptor scaffold/adaptor protein
          参与通路
          cell cycle cell adhesion transmembrane receptor protein tyrosine kinase signaling pathway epidermal growth factor receptor signaling pathway cell differentiation osteoclast development peptidyl-tyrosine autophosphorylation regulation of cell proliferation innate immune response bone resorption progesterone receptor signaling pathway regulation of cell cycle negative regulation of extrinsic apoptotic signaling pathway negative regulation of intrinsic apoptotic signaling pathway stimulatory C-type lectin receptor signaling pathway signal transduction signal complex assembly transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway central nervous system development cell proliferation response to mechanical stimulus response to virus response to acidic pH regulation of epithelial cell migration positive regulation of epithelial cell migration positive regulation of platelet-derived growth factor receptor signaling pathway positive regulation of glucose metabolic process positive regulation of smooth muscle cell migration single organismal cell-cell adhesion peptidyl-serine phosphorylation peptidyl-tyrosine phosphorylation regulation of cell-cell adhesion platelet activation intracellular estrogen receptor signaling pathway forebrain development T cell costimulation protein destabilization response to nutrient levels positive regulation of protein autophosphorylation activation of protein kinase B activity negative regulation of telomere maintenance via telomerase negative regulation of protein homooligomerization cellular response to insulin stimulus regulation of intracellular estrogen receptor signaling pathway positive regulation of integrin activation adherens junction organization substrate adhesion-dependent cell spreading cellular response to reactive oxygen species intracellular signal transduction cellular response to platelet-derived growth factor stimulus Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway response to drug response to hydrogen peroxide positive regulation of apoptotic process negative regulation of apoptotic process regulation of vascular permeability stress fiber assembly negative regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of protein binding positive regulation of MAP kinase activity positive regulation of phosphatidylinositol 3-kinase activity transcytosis regulation of bone resorption positive regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of insulin receptor signaling pathway protein autophosphorylation platelet-derived growth factor receptor signaling pathway vascular endothelial growth factor receptor signaling pathway neurotrophin TRK receptor signaling pathway ephrin receptor signaling pathway oogenesis positive regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation leukocyte migration positive regulation of small GTPase mediated signal transduction response to mineralocorticoid response to electrical stimulus negative regulation of focal adhesion assembly positive regulation of protein kinase B signaling negative regulation of mitochondrial depolarization negative regulation of telomerase activity uterus development branching involved in mammary gland duct morphogenesis regulation of cell projection assembly positive regulation of ERK1 and ERK2 cascade response to interleukin-1 cellular response to lipopolysaccharide cellular response to peptide hormone stimulus cellular response to progesterone stimulus cellular response to fatty acid cellular response to hypoxia cellular response to fluid shear stress regulation of podosome assembly positive regulation of podosome assembly positive regulation of protein serine/threonine kinase activity angiotensin-activated signaling pathway involved in heart process positive regulation of canonical Wnt signaling pathway positive regulation of lamellipodium morphogenesis positive regulation of DNA biosynthetic process regulation of early endosome to late endosome transport negative regulation of anoikis regulation of caveolin-mediated endocytosis immune system process protein phosphorylation positive regulation of gene expression phosphorylation cell migration positive regulation of cell adhesion positive regulation of protein transport cellular response to transforming growth factor beta stimulus positive regulation of intracellular signal transduction
          It has to be done as per old AB suggested Products section.
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