Invitrogen

Phospho-Stat5 (Tyr694) Recombinant Rabbit Monoclonal Antibody (Stat5Y694-B5)

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问题与答案

问题与答案

抗体检测数据 (4)

Phospho-Stat5 (Tyr694) Antibody in Flow Cytometry (Flow)

Phospho-Stat5 (Tyr694) Antibody in Flow Cytometry (Flow)

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Phospho-Stat5 (Tyr694) Antibody (MA5-37061) in Flow

Flow cytometry of Phospho-Stat5 (Tyr694) using K562 cells. Samples were incubated in monoclonal Phospho-Stat5 (Tyr694) antibody (Product # MA5-37061) with a dilution of 0.1 µg/mL. Images correspond with the following conditions: negative control (light blue), treated with imatinib (red), pervanadate-treated (green), imatinib and blocked with phospho-peptide (black), pervanadate and blocked with phospho pept... View More

Phospho-Stat5 (Tyr694) Antibody in Flow Cytometry (Flow)

Phospho-Stat5 (Tyr694) Antibody in Flow Cytometry (Flow)

Phospho-Stat5 (Tyr694) Antibody in Flow Cytometry (Flow)

Phospho-Stat5 (Tyr694) Antibody in Flow Cytometry (Flow)

Product Image Gallery

Phospho-Stat5 (Tyr694) Antibody in Flow Cytometry (Flow) — Flow cytometry of Phospho-Stat5 (Tyr694) using K562 cells. Samples were incubated in monoclonal Phospho-Stat5 (Tyr694) antibody (Product # MA5-37061) with a dilution of 0.1 µg/mL. Images correspond with the following conditions: negative control (light blue), treated with imatinib (red), pervanadate-treated (green), imatinib and blocked with phospho-peptide (black), pervanadate and blocked with phospho peptide (gold), imatinib and blocked with non-phospho peptide (dark blue), pervanadate and blocked with non-phospho peptide (purple).

Phospho-Stat5 (Tyr694) Antibody in Flow Cytometry (Flow) — Flow cytometry of Phospho-Stat5 (Tyr694) using NIH3T3 cells. Samples were incubated in monoclonal Phospho-Stat5 (Tyr694) antibody (Product # MA5-37061) with a dilution of 0.1 µg/mL. Images correspond with the following conditions: negative control (blue), control (orange), treated with imatinib (red), with pervanadate (green).

Phospho-Stat5 (Tyr694) Antibody in Flow Cytometry (Flow) — Flow cytometry of Phospho-Stat5 (Tyr694) using K562 cells. Samples were incubated in monoclonal Phospho-Stat5 (Tyr694) antibody (Product # MA5-37061) with a dilution of 0.05 µg/mL. Images correspond with the following conditions: negative control (blue), treated with imatinib (red), treated with pervanadate (green).

Phospho-Stat5 (Tyr694) Antibody in Flow Cytometry (Flow) — Flow cytometry of Phospho-Stat5 (Tyr694) using 3T3 cells. Samples were incubated in monoclonal Phospho-Stat5 (Tyr694) antibody (Product # MA5-37061) with a dilution of 0.1 µg/mL. Images correspond with the following conditions: negative control (blue), treated with imatinib (red), with pervanadate (green).

Immunogen image

Phospho-Stat5 (Tyr694) Recombinant Rabbit Monoclonal Antibody (Stat5Y694-B5)

Immunogen Image Phospho-Stat5 (Tyr694) Recombinant Rabbit Monoclonal Antibody (Stat5Y694-B5)

产品信息

MA5-37061

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1-1,000 ng/mL

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG, kappa

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

Stat5Y694-B5

抗原

A synthetic phospho-peptide corresponding to residues surrounding Tyr694 of human phospho Stat5.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A/G

保存液

PBS with 0.10% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5 alpha is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of STAT5 alpha is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells. STAT5 alpha, along with STAT5 beta, were identified in mouse. The amino acid sequences of STAT5 alpha and STAT5 beta show 96% sequence similarity, and both proteins are co expressed in most tissues in virgin and lactating mice. However, differential accumulation of STAT5 alpha and STAT5 beta mRNA has been reported for both muscle and mammary tissue. STAT5 alpha is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Mammary gland factor; mammary gland factor STAT5A; Signal transducer and activator of transcription 5A; STAT; STAT5B

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基因别名: AA959963; MGF; Mpf; STAT5; STAT5A

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UniProt ID: (Human) P42229, (Mouse) P42230

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Entrez Gene ID: (Human) 6776, (Mouse) 20850

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DNA binding transcription factor activity, sequence-specific DNA binding protein tyrosine kinase activity signal transducer activity protein binding RNA polymerase II core promoter sequence-specific DNA binding double-stranded DNA binding protein phosphatase binding sequence-specific DNA binding DNA-binding transcription factor

allantoin metabolic process positive regulation of endothelial cell proliferation citrate metabolic process 2-oxoglutarate metabolic process succinate metabolic process oxaloacetate metabolic process transcription, DNA-templated regulation of transcription from RNA polymerase II promoter isoleucine metabolic process valine metabolic process creatine metabolic process fatty acid metabolic process JAK-STAT cascade lactation peptidyl-tyrosine phosphorylation taurine metabolic process prolactin signaling pathway regulation of multicellular organism growth positive regulation of blood vessel endothelial cell migration creatinine metabolic process JAK-STAT cascade involved in growth hormone signaling pathway luteinization natural killer cell differentiation regulation of transcription, DNA-templated transcription from RNA polymerase II promoter signal transduction female pregnancy positive regulation of cell proliferation regulation of steroid metabolic process cytokine-mediated signaling pathway lipid storage regulation of cell adhesion regulation of epithelial cell differentiation mammary gland development positive regulation of natural killer cell differentiation negative regulation of mast cell apoptotic process T cell differentiation in thymus positive regulation of multicellular organism growth positive regulation of activated T cell proliferation T cell homeostasis negative regulation of apoptotic process response to peptide hormone positive regulation of interleukin-2 biosynthetic process positive regulation of B cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of lymphocyte differentiation negative regulation of erythrocyte differentiation positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter positive regulation of natural killer cell mediated cytotoxicity development of secondary female sexual characteristics development of secondary male sexual characteristics Peyer's patch development positive regulation of inflammatory response mammary gland involution positive regulation of mast cell differentiation prostate gland epithelium morphogenesis epithelial cell differentiation involved in prostate gland development mammary gland epithelium development positive regulation of mast cell proliferation cellular response to granulocyte macrophage colony-stimulating factor stimulus