Invitrogen

Phospho-TRPM7 (Ser1513) Polyclonal Antibody

Name: Phospho-TRPM7 (Ser1513) Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-143779

查看其他22个TRPM7抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

Phospho-TRPM7 (Ser1513) Antibody in Western Blot (WB)

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Phospho-TRPM7 (Ser1513) Antibody (PA5-143779) in WB

Western blot of Phospho-TRPM7 (Ser1513) in human auto phosphorylated TRPM7 C-terminal kinase domain (lanes 1-6). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 phosphosites (lanes 2, 4, & 6). The blot was probed with rabbit Phospho-TRPM7 (Ser1513) polyclonal antibody (Product # PA5-143779) (lanes 1 & 2), anti-TRPM7 (a.a.1484-1497) antibody (lanes 3 & 4), or anti-TRPM7 (Ser1493), (lane... View More

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产品信息

PA5-143779

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Phospho-TRPM7 (Ser-1513) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Ser-1513 in human TRPM7. This site is well conserved in rat and mouse TRPM7, but has low homology to other TRPM family members.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA, 50% glycerol

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2943007

产品详细信息

The antibody was cross-adsorbed to unphosphorylated TRPM7 (Ser-1513) peptide then affinity purified using phospho-TRPM7 (Ser-1513) peptide. This antibody detects a 220 kDa* protein on SDS-PAGE immunoblots of human MDA-MB-231 and rat PC12 cells stimulated with calyculin A, as well as detects a 70 kDa autophosphorylated recombinant kinase domain of human TRPM7. These reactivities are not observed after lambda phosphatase treatment to dephosphorylate TRPM7.

靶标信息

TRPCs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Channel kinase 1; Channel-kinase 1; FLJ20117; Long transient receptor potential channel 7; long transient receptor potential channel 7 {ECO:0000250|UniProtKB:Q96QT4}; LTRPC ion channel family member 7; LTrpC-7; LTrpC7 {ECO:0000250|UniProtKB:Q96QT4}; tr; Transient receptor potential cation channel subfamily M member 7; transient receptor potential cation channel subfamily M member 7 {ECO:0000312|RGD:620053}; transient receptor potential cation channel, subfamily M, member 7; transient receptor potential M7; Transient receptor potential-phospholipase C-interacting kinase; transient receptor potential-phospholipase C-interacting kinase {ECO:0000303|PubMed:11161216}; transient receptor potential-related protein, ChaK; TRMP7; TRP PLIK; TRP-PLIK; TRP-PLIK {ECO:0000303|PubMed:11161216}; trpm7 {ECO:0000312|RGD:620053}

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基因别名: 2310022G15Rik; 4833414K03Rik; 5033407O22Rik; ALSPDC; CHAK; CHAK1; Ltpr7; LTrpC-7; LTRPC7; TRP-PLIK; TRPM7; TRPPLIK

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UniProt ID: (Human) Q96QT4, (Mouse) Q923J1, (Rat) Q925B3

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Entrez Gene ID: (Human) 54822, (Mouse) 58800, (Rat) 679906

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功能