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Invitrogen
Phospho-VE-cadherin (Tyr658) Polyclonal Antibody
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Phospho-VE-cadherin (Tyr658) Antibody (44-1144G) in WB
Extracts of HUVEC cells either untreated (1) or treated (2-5)with 100 ng/mL VEGF for 10. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with the VE-cadherin (pY658) (Product # 44-1144G).
产品信息
44-1144G
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Bovine,
C. elegans,
Hamster,
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human VE-cadherin that contains tyrosine 658. This sequence is conserved in chicken, chimpanzee, cow, dog, mouse, pig, and rat.
偶联物
形式
Liquid
纯化类型
Antigen affinity chromatography
保存液
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated VE-cadherin. The final product is generated by affinity chromatography using an VE-cadherin-derived peptide that is phosphorylated at tyrosine 658.
Positive controls used with this antibody in western blotting were HUVEC +/- VEGF.
靶标信息
VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer. VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 7B4 antigen; 7B4/cadherin-5; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144; cd144 antigen; endothelial-specific cadherin; Vascular endothelial cadherin; VE-cadherin
基因别名: 7B4; AA408225; CD144; CDH5; VE-Cad; Vec; VEcad; VECD
UniProt ID: (Human) P33151, (Mouse) P55284
Entrez Gene ID: (Human) 1003, (Mouse) 12562
blood vessel maturation
cell-cell junction assembly
homophilic cell adhesion via plasma membrane adhesion molecules
transforming growth factor beta receptor signaling pathway
negative regulation of cell proliferation
single organismal cell-cell adhesion
adherens junction organization
positive regulation of establishment of endothelial barrier
regulation of establishment of cell polarity
cell adhesion
