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Invitrogen

Phospho-c-Kit (Tyr730) Polyclonal Antibody

Advanced Verification
2 Published Figures
4 References
View all (167) c-Kit (CD117) antibodies

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Phospho-c-Kit (Tyr730) Polyclonal Antibody

Product Details

44-496G

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50
-
Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Kit that contains tyrosine 730. The sequence is conserved in mouse, rat and cow.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533666

Target Information

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: belly-spot; c-Kit; c-KIT gene1; c-kit proto-oncogene protein; c-kit receptor; c-kit receptor tyrosine kinase; CD117; ckit; dominant spotting; Dominant white spotting; mast cell growth factor receptor; Mast/stem cell growth factor receptor Kit; p145 c-kit; PBT; Piebald trait protein; protein kinase; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; receptor tyrosine kinase c-kit; receptor tyrosine kinase type III; SCFR; spotted sterile male; Steel Factor Receptor; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

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Gene Aliases: Bs; c-KIT; CD117; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; KIT; SCFR; SCO1; SCO5; Sl; SOW3; Ssm; Tr-kit; W

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UniProt ID: (Dog) O97799, (Chicken) Q08156, (Mouse) P05532

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Entrez Gene ID: (Bovine) 280832, (Dog) 403811, (Chicken) 378783, (Rat) 64030, (Mouse) 16590

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Function(s)
transmembrane receptor protein tyrosine kinase activity ATP binding cytokine binding metal ion binding protease binding stem cell factor receptor activity SH2 domain binding protein homodimerization activity protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity nucleotide binding protein kinase activity protein binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups transmembrane signal receptor
Process(es)
activation of MAPK activity ovarian follicle development immature B cell differentiation dendritic cell cytokine production inflammatory response spermatogenesis regulation of cell shape peptidyl-tyrosine phosphorylation cytokine-mediated signaling pathway lamellipodium assembly T cell differentiation erythrocyte differentiation melanocyte differentiation actin cytoskeleton reorganization mast cell cytokine production embryonic hemopoiesis megakaryocyte development Fc receptor signaling pathway Kit signaling pathway erythropoietin-mediated signaling pathway mast cell degranulation pigmentation digestive tract development stem cell differentiation detection of mechanical stimulus involved in sensory perception of sound cell chemotaxis mast cell differentiation melanocyte migration melanocyte adhesion hematopoietic progenitor cell differentiation myeloid progenitor cell differentiation lymphoid progenitor cell differentiation mast cell chemotaxis glycosphingolipid metabolic process transmembrane receptor protein tyrosine kinase signaling pathway multicellular organism development spermatid development positive regulation of cell proliferation male gonad development response to radiation positive regulation of gene expression B cell differentiation positive regulation of cell migration positive regulation of kinase activity ectopic germ cell programmed cell death intracellular signal transduction hematopoietic stem cell migration positive regulation of tyrosine phosphorylation of STAT protein negative regulation of programmed cell death positive regulation of MAP kinase activity positive regulation of JAK-STAT cascade protein autophosphorylation positive regulation of sequence-specific DNA binding transcription factor activity cellular response to thyroid hormone stimulus positive regulation of vascular smooth muscle cell differentiation myeloid leukocyte differentiation protein phosphorylation germ cell migration visual learning signal transduction by protein phosphorylation hemopoiesis positive regulation of pseudopodium assembly somatic stem cell population maintenance positive regulation of tyrosine phosphorylation of Stat1 protein positive regulation of tyrosine phosphorylation of Stat3 protein positive regulation of tyrosine phosphorylation of Stat5 protein positive regulation of MAPK cascade positive regulation of Notch signaling pathway developmental pigmentation regulation of developmental pigmentation somatic stem cell division positive regulation of long-term neuronal synaptic plasticity epithelial cell proliferation chemotaxis germ cell development phosphorylation cell differentiation
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