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Invitrogen
Phospho-p27 Kip1 (Ser10) Polyclonal Antibody
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Phospho-p27 Kip1 (Ser10) Antibody (34-6300) in WB
Western blot analysis of recombinant purified wild-type p27 and p27 (S10A) mutants after phosphorylation in vivo by either cyclin E-Cdk2 (lanes 1 and 2) or ERK2 (lanes 3 and 4) using Rb anti-phospho-p27 (Ser10) (Product # 34-6300) (top), Rb anti-phospho-p27 (Thr187) (Product # 71-7700) (middle), and a monoclonal antibody to p27 (bottom). Image reproduced from Rodier G, et al. EMBO J 2001.
产品信息
34-6300
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide encompassing the phosphorylated Ser10 residue of p27/Kip1.
偶联物
形式
Liquid
浓度
0.25 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
0.1% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标信息
p27 Kip1 functions as a negative regulator of G1 mouse expression and has been proposed to function as a possible mediator of TGFb induced G1 arrest. p27 Kip1 is an important regulator of cell cycle progression, a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. p27 Kip1 acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. p27 Kip1 has a molecular weight of approximately 27kDa and the degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CDN1B; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor 1B (p27 Kip1); Cyclin-dependent kinase inhibitor 1B (p27, Kip1); Cyclin-dependent kinase inhibitor p27; Cyclin-dependent kinase inhibitor p27 Kip1; cyclin-dependent kinase inhibitor p27/Kip1; P27 CDKN1B; p27(KIP1); p27Kip1
基因别名: AA408329; AI843786; Cdki1b; CDKN1B; CDKN4; KIP1; MEN1B; MEN4; p27; P27KIP1
UniProt ID: (Human) P46527, (Mouse) P46414
Entrez Gene ID: (Human) 1027, (Mouse) 12576, (Rat) 83571
cyclin-dependent protein serine/threonine kinase inhibitor activity
transforming growth factor beta receptor, cytoplasmic mediator activity
protein binding
cysteine-type endopeptidase activator activity involved in apoptotic process
protein phosphatase binding
Hsp70 protein binding
protein complex binding
chaperone binding
protein kinase inhibitor activity
kinase activity
kinase inhibitor
regulation of cyclin-dependent protein serine/threonine kinase activity
G1/S transition of mitotic cell cycle
response to hypoxia
potassium ion transport
activation of cysteine-type endopeptidase activity involved in apoptotic process
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
cell cycle arrest
Notch signaling pathway
sensory perception of sound
positive regulation of cell proliferation
negative regulation of cell proliferation
response to glucose
positive regulation of cell death
negative regulation of cell growth
positive regulation of microtubule polymerization
response to estradiol
negative regulation of kinase activity
negative regulation of phosphorylation
response to drug
negative regulation of apoptotic process
response to amino acid
response to peptide hormone
positive regulation of protein catabolic process
negative regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of cell cycle
negative regulation of transcription, DNA-templated
negative regulation of mitotic cell cycle
response to cadmium ion
autophagic cell death
inner ear development
negative regulation of cellular component movement
negative regulation of epithelial cell proliferation involved in prostate gland development
cellular response to antibiotic
cellular response to lithium ion
cellular response to organic cyclic compound
mitotic cell cycle arrest
negative regulation of vascular smooth muscle cell proliferation
cell cycle
cell death
regulation of cell proliferation
negative regulation of epithelial cell proliferation
response to organic cyclic compound
phosphorylation
