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Invitrogen
Phospholamban Polyclonal Antibody
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Phospholamban Antibody (PA5-94957) in WB
Western blot analysis of PLN in, Lane 1: rat heart tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse heart tissue lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. The membrane was blocked with 5% non-fat milk/TBS for 1.5 hour a... View More
产品信息
PA5-94957
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the N-terminus of human PLN (2-16aa EKVQYLTRSAIRRAS).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide, 0.05mg thimerosal
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat heart tissue, rat heart tissue, mouse heart tissue, mouse heart tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: cardiac CMH18; cardiac phospholamban; Phospholamban; PLB
基因别名: CMD1P; CMH18; PLB; Plm; PLN
UniProt ID: (Human) P26678, (Rat) P61016, (Mouse) P61014
Entrez Gene ID: (Human) 5350, (Rat) 64672, (Mouse) 18821
regulation of the force of heart contraction
calcium ion transport
cellular calcium ion homeostasis
regulation of heart contraction
response to zinc ion
negative regulation of heart rate
regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
negative regulation of ATPase activity
response to insulin
response to testosterone
negative regulation of heart contraction
cardiac muscle tissue development
protein homooligomerization
regulation of calcium ion transport
negative regulation of calcium ion transport
regulation of ryanodine-sensitive calcium-release channel activity
regulation of cardiac muscle cell contraction
adrenergic receptor signaling pathway involved in heart process
regulation of the force of heart contraction by cardiac conduction
regulation of calcium ion import
negative regulation of calcium ion transmembrane transporter activity
regulation of relaxation of muscle
negative regulation of calcium ion binding
regulation of calcium-transporting ATPase activity
negative regulation of calcium-transporting ATPase activity
Notch signaling pathway
negative regulation of catalytic activity
blood circulation
regulation of cytosolic calcium ion concentration
relaxation of cardiac muscle
regulation of cardiac muscle cell membrane potential
negative regulation of calcium ion import
regulation of relaxation of cardiac muscle
negative regulation of calcium ion import into sarcoplasmic reticulum
regulation of cardiac conduction
