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Agrisera
PhyB/Phytochrome B (dicots) Polyclonal Antibody
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PhyB/Phytochrome B (dicots) Antibody (AS21-4566) in WB
Samples: Ler - 30 µg of Arabidopsis thaliana Ler dark grown seedling extract phy B-5 - 30 µg of Arabidopsis thaliana phy B-5 mutant dark grown seedling extract MW markers: Page Ruler Prestained Protein Ladder (Thermo Fisher Scientific) 30 µg/well of total protein extracted from 4-days-old Arabidopsis thaliana seedlings with extraction buffer (65 m M Tris/HCl p H 6,8; 4 M Urea; 10% glycerol; 3% SDS; 0.05% br... View More
产品信息
AS21-4566
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana PhyB, C-terminal.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
Phytochrome B is a red/far-red light-sensing photoreceptor that accumulates in the cytosol in darkness predominantly in the inactive Pr form and, upon red light conversion to the active Pfr form, translocates into the nucleus where it concentrates in nuclear photobodies and initiates transcriptional reprogramming. It is a soluble dimeric chromoprotein with an N-terminal photosensory module that binds a linear tetrapyrrole chromophore and contains conserved PAS-GAF-PHY subdomains responsible for photoconversion, coupled to a C-terminal regulatory module bearing histidine kinase-related signaling motifs and a nuclear localization activity that supports light-dependent nuclear accumulation. Biologically, phytochrome B functions as a central light-quality and light-quantity sensor controlling photomorphogenesis, including de-etiolation and shade avoidance, largely by directly engaging PHYTOCHROME INTERACTING FACTOR (PIF) bHLH transcription factors in the nucleus to promote their rapid inhibition through sequestration and/or ubiquitin-proteasome-mediated turnover, thereby modulating downstream gene expression programs and growth responses.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT2G18790; HY3; MSF3.17; MSF3_17; OOP1; OUT OF PHASE 1; PHYB; PHYTOCHROME B
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