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Bethyl Laboratories
Pol kappa Polyclonal Antibody
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Pol kappa Antibody (A301-976A) in IP
Detection of human Pol kappa by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Pol kappa antibody (Product # A301-976A) used for IP at 3 µg/mg lysate. Pol kappa was also immunoprecipitated by rabbit anti-Pol kappa antibodies A301-975A and A301-977A, which recognize other epitopes. For blotting immunoprecipitated Pol kappa, A301-975A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
产品信息
A301-976A
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Region between residue 775 and 825 of Human DNA polymerase kappa.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
tris citrate/phosphate, pH 7-8
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
The recommended shelf life for this product is 1 year from date of receipt.
靶标信息
External and internal DNA-damaging agents continually threaten the integrity of genetic material in cells. Although a variety of repair mechanisms exist to remove the resulting lesions, some lesions escape repair and block the replication machinery. Members of the Y family of DNA polymerases, such as POLK, permit the continuity of the replication fork by allowing replication through such DNA lesions. Each Y family polymerase has a unique DNA-damage bypass and fidelity profile. POLK is specialized for the extension step of lesion bypass.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DINB protein; DINP; DNA polymerase kappa; polymerase (DNA directed) kappa
基因别名: DINB1; DINP; POLK; POLQ
UniProt ID: (Human) Q9UBT6
Entrez Gene ID: (Human) 51426
