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Bethyl Laboratories
Pragmin Polyclonal Antibody
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Pragmin Antibody (A302-675A) in WB
Detection of human Pragmin by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Pragmin antibody (Product # A302-675A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. Pragmin was also immunoprecipitated by rabbit anti-Pragmin antibody BL9745, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
产品信息
A302-675A
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Region between residue 275 and 325 of Human homolog of rat pragma of Rnd2.
偶联物
形式
Liquid
浓度
0.20 mg/mL
纯化类型
Antigen affinity chromatography
保存液
TBS, pH 7.0 to 8.0, with 0.1% BSA
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
The recommended shelf life for this product is 1 year from date of receipt.
靶标信息
PRAGMIN is also known as SGK223 (sugen kinase 223). The rat homolog was originally identified in a yeast two-hybrid screen for Rnd2 effector proteins expressed in neural cells. Rat Pragmin was found to stimulate RhoA activity for the regulation of neurite outgrowth.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Inactive tyrosine-protein kinase PRAG1; PEAK1-related kinase-activating pseudokinase 1; Pragmin; SgK223; Sugen kinase 223
基因别名: PRAG1; PRAGMIN; SGK223
UniProt ID: (Human) Q86YV5
Entrez Gene ID: (Human) 157285
