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Invitrogen
Prohibitin Recombinant Rabbit Monoclonal Antibody (SR46-02)
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图: 1 / 15
Prohibitin Antibody (MA5-32000) in ICC/IF
Immunofluorescence analysis of Prohibitin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Prohibitin Recombinant Rabbit Monoclonal Antibody (SR46-02) (Product # MA5-32000) at 1:100 dilution in 0.1% ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA5-32000
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat,
Zebrafish
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
SR46-02
抗原
Synthetic peptide within Human Prohibitin aa 147-196.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
Prohibitin is from an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: B-cell receptor-associated protein 32; BAP 32; OTTHUMP00000165765; OTTHUMP00000165766; OTTHUMP00000220103; OTTHUMP00000220170; OTTHUMP00000220171; prohibitin; Prohibitin 1
基因别名: Bap32; fa18f07; fb09f05; PHB; PHB1; sr:nyz128; wu:fa18f07; wu:fb09f05
UniProt ID: (Mouse) P67778, (Rat) P67779
Entrez Gene ID: (Mouse) 18673, (Rat) 25344, (Zebrafish) 321346
RNA polymerase II transcription factor activity, sequence-specific DNA binding
complement component C3a binding
complement component C3b binding
protein C-terminus binding
enzyme binding
proteinase activated receptor binding
histone deacetylase binding
transcription regulatory region DNA binding
molecular_function
negative regulation of transcription from RNA polymerase II promoter
osteoblast differentiation
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
mitochondrion organization
negative regulation of cell proliferation
negative regulation of transcription by competitive promoter binding
histone deacetylation
negative regulation of cell growth
negative regulation of protein catabolic process
negative regulation of apoptotic process
positive regulation of G-protein coupled receptor protein signaling pathway
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of complement activation
protein stabilization
progesterone receptor signaling pathway
negative regulation of androgen receptor signaling pathway
negative regulation of ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
cellular response to interleukin-6
DNA biosynthetic process
negative regulation of glucocorticoid receptor signaling pathway
ovarian follicle development
ovarian follicle atresia
organ regeneration
response to cytokine
response to immobilization stress
response to drug
response to peptide hormone
response to ethanol
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