Invitrogen

Prolactin Receptor Monoclonal Antibody (1A2B1)

Name: Prolactin Receptor Monoclonal Antibody (1A2B1)
Brand: Invitrogen
SKU: 35-9200

高级验证

34个已发表图片

47 篇参考文献

查看其他34个Prolactin Receptor抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Prolactin Receptor Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 37

Prolactin Receptor Antibody (35-9200) in ICC/IF

Immunofluorescence analysis of Prolactin Receptor was performed using 70% confluent log phase SW40 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Prolactin Receptor (ECD) (1A2B1) Monoclonal Antibody (Product # 35-9200) at 2µg/mL in 0.1% BSA and incub... View More

Prolactin Receptor Antibody in Western Blot (WB)

Prolactin Receptor Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Prolactin Receptor Antibody in Immunohistochemistry (IHC)

Prolactin Receptor Antibody in Western Blot, Flow Cytometry (WB, Flow)

Prolactin Receptor Antibody in Western Blot, Immunoprecipitation (WB, IP)

Prolactin Receptor Antibody in Proximity Ligation Assay (PLA) (PLA)

Product Image Gallery

产品信息

35-9200

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

查看22篇已发表文章 22篇已发表文章

免疫组化 (IHC)

5-10 µg/mL

查看8篇已发表文章 8篇已发表文章

免疫组化（石蜡） (IHC (P))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

2 µg/mL

查看5篇已发表文章 5篇已发表文章

流式细胞分析 (Flow)

查看2篇已发表文章 2篇已发表文章

免疫沉淀 (IP)

5-10 µg/mL

查看3篇已发表文章 3篇已发表文章

中和实验 (Neu)

查看1篇已发表文章 1篇已发表文章

Proximity Ligation Assay (PLA) (PLA)

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

查看4篇已发表文章 4篇已发表文章

产品规格

种属反应

Human

已发表种属

Hamster, Human, Mouse

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

1A2B1

抗原

Recombinant human prolactin receptor protein containing the extracellular domain (ECD).

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533231

产品详细信息

This antibody is predicted to react with mouse and rat and reacts with the long form of the human prolactin receptor (hPRLr) protein at ~85-90 kDa, and may also identify the human prolactin receptor intermediate and delta S1 isoforms by western blotting. On immunoprecipitation, this antibody strongly recognizes only the long form of hPRLr. During development reactivity was confirmed with human T-47D breast carcinoma cells.

靶标信息

Prolactin (PRL) is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor (PRLR), a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. PRLR consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

仅用于科研。不用于诊断过程。未经明确授权不得转售。