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PsaD/PSI-D subunit of photosystem I Polyclonal Antibody
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PsaD/PSI-D subunit of photosystem I Antibody (AS09-461) in WB
Total cellular (lanes 2 – 5) and membrane proteins (lanes 6 – 9) from various environmental isolated of Chlamydomonas reinhardtii were extracted with a buffer containing 62.5 m M Tris-HCl p H 6.8,10% glycerol, 2% SDS, 50 m M DTT, 10 m M Na F and 1% protease inhibitors (P 9599, Sigma Aldrich) and denatured at 65°C for 5 min. Samples (0.25 µg of chlorophyll per lane) were separated on 12% SDS-PAGE containing ... View More
产品信息
AS09-461
产品规格
种属反应
Algae,
Bacteria,
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide 100% conserved in all known plant PsaD sequences.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Dioxoniella giordanoi (red alga), Hordeum vulgare, Lactuca sativa, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Picea glauca, Pinus strobus, Spinacia oleracea, Synechocystis PCC 6803, Triticum aestivum, Triticale, Zea mays
靶标信息
psaD1 encodes a nuclear-encoded, chloroplast-targeted photosystem I (PSI) reaction center subunit II-1 (PSI-D), a small (~208 aa precursor) protein imported into chloroplasts and localized on the stromal side of the thylakoid membrane where it associates peripherally with the PSI core. The mature protein is hydrophilic and enriched in basic residues that promote stable binding to the PSI complex and formation of a ferredoxin-docking interface; structural modeling and comparative biochemistry support a compact, largely alpha-helical fold typical of PsaD proteins that contacts other PSI subunits (including PsaB, PsaC, and PsaL) to stabilize the stromal ridge of PSI. Functionally, PSI-D is required for efficient electron transfer from PSI to soluble ferredoxin and thereby supports downstream NADP+ photoreduction; genetic analyses in Arabidopsis show that loss of both PSI-D isoforms is seedling-lethal and eliminates accumulation of PSI subunits, while disruption of psaD1 alone reduces PsaD abundance, impairs photosynthetic electron flow, slows growth, and triggers broad downregulation of light-reaction gene expression, indicating an essential role for psaD1 in PSI assembly/stability and maintenance of photosynthetic capacity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: photosystem I reaction center subunit II-1; photosystem I subunit D1; PSAD1; PSI-D1
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